ANTIBIOTIC SUSPECTIBILITY PATTERN OF VARIOUS BACTERIAL AND FUNGAL SPECIES ISOLATED FROM BLOOD CULTURE OF CHILDREN- A PROSPECTIVE STUDY
Journal Title: Journal of Advanced Medical and Dental Sciences Research - Year 2017, Vol 5, Issue 3
Abstract
Background: Early administration of adequate antibiotic therapy has been shown to reduce mortality associated with bloodstream infection and has a positive impact on the outcome of bacteraemic patients. Septic shock is defined as sepsis associated with evidence of organ hypoperfusion and a systolic blood pressure <90 or >30 mm Hg less than the baseline or a requirement for the use of vasopressor to maintain the blood pressure. About 70% of Staphylococci isolated in blood cultures are resistant to Penicillin, the most effective antibiotic against the Staphylococcal isolate is Clindamycin (70%) followed by Vancomycin (40%). The aim of present study is to establish the antibiotic sensitivity pattern amongst various blood culture isolates obtained from neonatal blood cultures. Materials and methods: Department of microbiology and department of paediatrics of Integral Institute of Medical Sciences conducted a hospital based study involving 80 children and neonates.The ethical committee clearance was obtained prior to initiating the study and parents of all the subjects were informed about the study and a written consent was obtained from all.By using a sterile syringe blood was drawn through venipuncture before administration of antibiotics. Blood cultures were done by Bactec blood culture technique. Smears were prepared from positive blood cultures and examined. After cultures, bacteria were obtained in pure subcultures. The organism was isolated in pure culture on a solid medium. Isolated colonies was inoculated in a suitable broth medium and incubated at 35-370C for 4-6 hours. The density of the organism in broth was adjusted to approximately106 cfu/ml by comparing it’s turbidity with 0.5 McFarland opacity standard tubes. The antimicrobial susceptibility testing was done by Kirby-Bauer’s Disk Diffusion. The data thus obtained was arranged in a tabulated form. It was expressed as percentage of the total. Results: Out of the total of 80 newborns and children, 40% were females and 60% were males. S.aureus were more sensitive to neticllin, vancomycin, teicoplanin, linezolid & tigecycline(100%) followed by amikacin & tobramycin(93.75%). Coagulase negative Staphyolococci was found to be highly sensitive to vancomycin (100%), teicoplanin (100%), linezolid, netillin, tobramycin, tetracycline & tegicycline (100%) followed by clindamicin 80% and complete resistance (100 %) was seen with penicillin while 80% was seen in cefoxitin, amoxyclav, ciprofloxacin & ofloxacin. Candida albicans shows maximum sensitivity pattern to voriconazole (100%), itraconazole, fluconazole (85.71%) followed by amphotercin –B (57.14%). Conclusion: Staphylococcus aureus was found to be highly sensitive to vancomycin, teicoplanin, linezolid, netillin & tegicycline. Enterococcus was highly sensitive to tetracycline.E.coli was more sensitive to piperacillin/tazobactam, tegicycline and imipenem/cilastin. Candida albicans shows maximum sensitivity pattern to voriconazole.
Authors and Affiliations
Abhishek Jaiswal, Aditya Mishra, Rajesh Kumar Yadav, L. Agarwal
Keywords
Related Articles
- EP ID EP557780
- DOI -
- Views 122
- Downloads 0
PEDIATRIC NEPHROLOGY IN CASE OF UNDERNOURISHED CHILDREN RECOVERED FROM UNDERNUTRITION
Background- Adults born with a low birth weight due to exposure of the mother at mid gestation to famine during the B Hunger Winter of 1944–1945 have been found to have a 3.2-fold increased occurrence of albuminuria comp...
Assessment of Frequency of Occurrence of Malocclusion among Known Paediatric Population: A Clinical Study
Background: Malocclusion is one of the public health problem which effects growth and development of a child. Malocclusion can lead to dissatisfaction in esthetical appearance which can have a negative impact on child’s...
Estimation of Stature from Femur Length- A Forensic Study
Background: Stature estimation forms a major domain of medico-legal investigations used in the identification of unknown fragmentary and mutilated remains. The present study was conducted to assess the stature of person...
Microhardness of Flowable Bulk-Fill Composite Materials
Purpose: To evaluate the curing efficiency of various flowable bulk fill resin materials using Vickers hardness measurements and compare them to conventional resin-based composite materialaccording to the manufacturer re...
Periodontal Diseases and Systemic Conditions: A Comprehensive Review
Periodontal disease is highly prevalent, contributing to the global burden of chronic diseases and constituting a major public health problem. The relationship between periodontitis and other pathological conditions coul...