ANTIBIOTIC SYNERGY TEST: CHECKERBOARD METHOD ON MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2011, Vol 2, Issue 12
Abstract
Pseudomonas aeruginosa is a global emergence of multidrug resistant strains leading to a wide variety of nosocomial infections in humans. The clinical use of combination of antibiotic therapy for gram negative infections is probably more effective than monotherapy. Certain combinations of antibiotic exhibit synergistic antibacterial effects. Ampicillin and Kanamycin are the commonly used potent bactericidal antibiotics, active against gram negative bacteria and also act synergistically. Antibiotic susceptibility of Pseudomonas aeruginosa showed a high level of resistance with Optochin and Rifamycin. An intermediate effect was seen to Erythromycin and Chloramphenicol, Kanamycin and Ampicillin showed different minimum inhibitory concentration (MIC). Thus, the susceptible antibiotics were used in the checker board technique, which appears useful for determining antibiotic synergism against Pseudomonas aeruginosa.. The ΣFIC results showed synergy for concentrations of 80µg/ml and 40µg/ml of antibiotic combinations with fractional inhibitory concentration (FIC) of 0.48 at 37˚C over night incubation per ml. Thus, the combinations tested were bactericidal indicating the minimum inhibitory concentration and no growth illustrated the extensive activity of kanamycin which was enforced by the ampicillin resulting in an antibacterial effect.
Authors and Affiliations
Spoorthi Jain, Vishwanatha T, Reena V, Divyashree B. C, Aishwarya Sampath, Siddhalingeshwara K. G, Venugopal N, Ramesh I
Keywords
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