ANTICANCER ACTIVITY OF SILVER NANOBIOCONJUGATES SYNTHESISED FROM PIPER BETLE LEAVES EXTRACT AND ITS ACTIVE COMPOUND EUGENOL
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: Our present study deals with the anticancer property of silver nano bio conjugates synthesized from the methanolic extract of Piper betle leaves and the pure compound eugenol.Methods: The anticancer activity of the nano bio conjugates synthesized from the extract and pure compound, in comparison with their respective non-conjugated raw material, were studied using cancer cell lines. The anticancer activity was analyzed by cell viability and staining techniques. The cell viability was determined by the MTT assay and the apoptosis was evaluated by the AO/EtBr staining method, which was carried out in both the cancerous (oral carcinoma (KB) cell lines) and non-cancerous cells (primary cultured human buccal cells). The phase of cell death was assessed by the flow cytometry followed by the cytotoxicity assessment.Results: The results revealed that Piper betle leaves as well as their active phenolic compound eugenol exhibited strong anticancer activity in KB oral carcinoma cells. The anticancer effect of the extract was attributable to the component phenolics, eugenol respectively in betel leaves, as the phenolics also exhibited strong cytotoxicity in cancer cells. The anticancer activity increased by a marked magnitude when the extract/compound was administered as nano-bio conjugates. The significant observation was the differential effect evoked by the AgNPs, which was non-toxic to non-cancerous buccal cells while evoking a strong cytotoxicity in the cancer cells.Conclusion: The results proved that the phytoconstituents like phenols in the betel leaves play a major role in the reduction of nano bio conjugates. The anticancer properties of silver nano bio conjugates were higher in the conjugated nanoform than the non-conjugated form.
Authors and Affiliations
R. Preethi, P. R. Padma
Keywords
Related Articles
- EP ID EP576035
- DOI 10.22159/ijpps.2016.v8i9.12993
- Views 106
- Downloads 0
GREEN SYNTHESIS OF SILVER NANOPARTICLE FROM LEAF EXTRACT OF AEGLE MARMELOS AND EVALUATION OF ITS ANTIBACTERIAL ACTIVITY
Objective: The synthesis of metal nanoparticle is a growing area of research in modern material science and technology. Utilization of the silver nanoparticles in the field of biomedical nanotechnology and nanomedicines...
Validation of two spectrophotometric methods for fluoxetine quantification
Objective: This paper describes the development and validation of two UV-Visible spectrophotometric analysis methods, using absolute ethanol (method A) and HCl 0.1 M (method B) as solvents, to quantify fluoxetine (FLX) i...
EVALUATION OF ANTI-OBESITY ACTIVITY OF SOLASODINE IN HIGH FAT DIET-INDUCED OBESITY IN RAT
Objective: This study was planned to study the anti-obesity activities of solasodine on high fat (HF) diet-induced obese rats.Methods: Wistar rats were divided into six groups. Control group (Group 1) received normal die...
UMBELLIFERONE EXTENUATES THE ABNORMALITIES IN LIPID METABOLISM DURING D-GALACTOSAMINE AND LIPOPOLYSACCHARIDE INDUCED FULMINANT HEPATIC FAILURE IN RATS.
Objective: Coumarins and coumarin-related compounds are a plentiful source of potential drugs candidate in relation to its safety and efficacy. Hence, the present study was designed to evaluate the protective nature of U...
ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF BIOACTIVE COMPOUNDS PRODUCED BY STREPTOMYCES SP. KB1
Objective: To investigate the antimicrobial and cytotoxic activities of bioactive compounds produced by Streptomyces sp. KB1 isolated from the air sample at Ao-nang, Krabi province, Thailand.Methods: Streptomyces sp. KB1...