ANTICARIOGENIC POTENTIAL OF CAMELLIA SINENSIS ON STREPTOCOCCUS MUTANS- AN IN VITRO STUDY
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2019, Vol 8, Issue 16
Abstract
BACKGROUND Dental caries is one of the most common chronic diseases of modern times. Chemical substances used for prevention of dental caries are known to have many side effects. Thus, natural products should be explored for their anti-caries action. We wanted to investigate the in-vitro antibacterial effect of tea leaves extract on Streptococcus mutans. METHODS This In Vitro experiment was conducted in the laboratory settings of Sri Aurobindo College of Pharmacy, Indore. Two types of tea leaves, black and oolong of a commercially available brand were used for the study. Aqueous extracts of both the tea leaves was prepared by boiling in distilled water in a standardized manner. Two concentrations 50% and 100%, were prepared and tested for antistreptococcal activity. Institute of Microbial Technology (IMTECH), Chandigarh supplied the bacterial strain Streptococcus mutans (MTCC 497) in lyophilized form. S.mutans was made viable by transferring into brain heart infusion (BHI) broth followed by incubation. Previously smeared S.mutans agar plates were then inoculated by the aqueous extracts of both the teas. Antimicrobial efficacy was checked using agar well diffusion method. The plates were incubated, and the zone of inhibition was determined. A similar procedure was carried out with Chlorhexidine 0.2%, which served as positive control. Statistical analysis was done using descriptive statistics and analytical tests like ANOVA to test the significance among variables. RESULTS Oolong as well as black tea aqueous extracts (100%), after an incubation of 48 hours hindered the growth of Streptococcus mutans. The efficacy of 100% aqueous extract was greater than 50% aqueous extract of both the types of tea. The mean zone of inhibition of oolong tea leaves aqueous extract (100%) was 19.18 ± 0.90 which was significantly higher than black tea 13.35±0.55 but it was less than chlorhexidine (0.2%) 24.80 ± 1.78 (p value<0.001). CONCLUSIONS A greater inhibitory effect was seen in Oolong tea extracts (Aqueous extract) compared to black tea (aqueous extract) on the growth of S.mutans
Authors and Affiliations
Pubali Sarkar, Sandeep Kumar, Suranjita Das, Bhuvnesh Airen
Keywords
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- EP ID EP564497
- DOI 10.14260/jemds/2019/283
- Views 87
- Downloads 0
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