ANTIDIABETIC ACTIVITY AND TOXICOLOGICAL EVALUATION OF THE METHANOL-DICHLOROMETHANE ROOT BARK EXTRACT OF NAUCLEA DIDERRICHII (DE WILD) MERR
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 9
Abstract
Objective: In southeastern Nigeria, Nauclea diderrichii (De Wild) Merr is used in the treatment of a wide range of ailments including diabetes mellitus (DM). This study evaluates the antidiabetic activity and toxicological profile of the methanol-dichloromethane root bark extract of N. diderrichii in normoglycemic and alloxan-induced diabetic models.Methods: Dried root barks of N. diderrichii were extracted using methanol and dichloromethane (1:1) to obtain N. diderrichii extract (NDE). The acute and sub-chronic toxicity tests were performed using standard procedures. The effect on alanine aminotransferase (ALT) aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), packed cell volume (PCV), hemoglobin concentration (HB) and total white blood cell (TWBC) count was determined in the rats after treatment. In order to elucidate its antidiabetic mode of action, an oral glucose tolerance test (OGTT) was performed using glucose (2 g/kg) as substrate and alloxan (100 mg/kg; i. v.) induced diabetic model. Glibenclamide (GLI 0.2 mg/kg) was used as the reference standard drug.Results: The results indicated that the LD50 of the extract is>5000 mg/kg. ALT, AST, ALP PCV, HB and TWBC were insignificantly (p>0.05) different compared with the control. No significant changes were observed in the organ weights compared with the control. In the acute and prolonged antidiabetic study, NDE (100, 200 and 400 mg/kg) significantly reduced the blood glucose level (BGL) by 14.66, 18.9, 25.80% and 75.11, 80.24, 83.74% respectively. In comparison, GLI, when administered, reduced BGL by 38.18 and 92.86% respectively.Conclusion: N. diderrichii possesses antidiabetic activity with good toxicological profile.
Authors and Affiliations
Theophine C. Akunne, Bonaventure C. Obi, Martha N. Ofokansi, Patience C. Nwonu, Charles O. Okoli
Keywords
Related Articles
- EP ID EP575531
- DOI 10.22159/ijpps.2017v9i9.20556
- Views 59
- Downloads 0
PHYTOCHEMICAL EVALUATION AND PHARMACOLOGICAL ACTIVITY OF SYZYGIUM AROMATICUM: A COMPREHENSIVE REVIEW
Medicinal plants are generating an ever-increasing amount of interest due to the effectiveness, low cost and minimal side-effects associated with drugs derived from them. Clove (Syzygium aromaticum (L.) (Family Myrtaceae...
A PROSPECTIVE COMPARATIVE OBSERVATIONAL STUDY ON SAFETY, EFFICACY AND COST EFFECTIVENESS OF INSULIN AND THEIR ANALOGUES
Objective: The objective of this study was to compare safety, efficacy and cost effectiveness of insulin and their analogues when compared with human insulin in patients with type-2 diabetes mellitus.Methods: A prospec...
THE EFFECT OF THE MEDICATION “DICLOCORâ€, CONTAINING DICLOFENAC AND QUERCETIN, ON CLINICO-BIOCHEMICAL PARAMETERS IN RATS WITH OSTEOARTHRITIS
Objective: The aim of this study was to evaluate chondroprotective effect of a combined medication Diclocor in comparison with its active mono components (diclofenac sodium and quercetin).Methods: The study was conducted...
PROTECTIVE EFFECT OF L-CARNITINE AND BAKER YEAST SACCHAROMYCES CEREVISIAE AGAINST HEPATIC TOXICITY INDUCED BY VALPROATE AS ANTIEPILEPTIC DRUG IN RATS
Objective: The aim of this work was to investigate the protective role of L-carnitine and baker yeast (Saccharomyces cerevisiae) against the effect of sodium valproate (VPA) induced toxicity and oxidative stress in the l...
STUDY THE LEVEL OF AWARENESS AMONG SINDHI FAMILIES OF DURG, CHHATTISGARH (INDIA): A QUESTIONNAIRE BASED STATISTICAL STUDY
Objective. The present study has been done with the following aims and objectives in mind: i) To determine the level of knowledge or awareness about Thalassemia among Sindhi families in Durg, Chhattisgarh regarding the c...