Antidiabetic activity, polyphenols-based characterization and molecular interaction of extract of un-ripe pods of Vinca rosea cv. Pink
Journal Title: Jordan Journal of Pharmaceutical Sciences - Year 2022, Vol 15, Issue 2
Abstract
The study aimed to investigate extracts of un-ripe pods of Vinca rosea cv. Pink for antidiabetic activity and polyphenol-based characterization of extracts using HPLC. Moreover, the molecular interaction of the identified markers with the antidiabetic targets was explored using molecular-docking software. Different extracts of un-ripe pods were prepared and investigated using antidiabetic models such as glucose uptake by yeast cells, alpha amylase inhibition and haemoglobin-glycosylation inhibition assays. The most active extract was characterized by HPLC using chlorogenic, caffeic and ferrulic acids as an analytical markers. The identified markers were taken as ligands for molecular docking with pancreatic α-amylase, glycogen phosphorelase and hexokinase-Ι using 1-Click Docking Mcule Software, and finding hydrogen-bonding affinities by UCSF Chimera 1.12. Methanol extracts showed higher antidiabetic activity of 37.77, 71.16 and 53.52% inhibition of α-amylase assay, increase in glucose-uptake by yeast cells, and inhibition of Hb-glycosylation assay, respectively. The extract was found to contain 0.25 mg/g of chlorogenic and 0.11 mg/g caffeic acids. These markers were found to be good ligands of diabetes related targets. The results indicate that methanol extract has antidiabetic activity, which may be assigned to chlorogenic and caffeic acids. These compounds may be used as pharmacological markers to standardize the extracts.
Authors and Affiliations
Abida Qamar
In-vitro Assessment of Essential Oils as Anticancer Therapeutic Agents: A Systematic Literature Review
Cancer is a fatal disease that causes around 9 million deaths annually in developing and developed countries worldwide. Recently, natural products as alternatives for chemical agents have become a growing area of intere...
Ethnopharmacological importance of local flora in the traditional medicine of Jordan: (A mini review)
Traditional knowledge of medicinal plant use in Jordan is poorly described. During the last years, many phytochemists and botanists from Jordan have conducted qualitative and quantitative several studies, to gather info...
Investigation of the Chemical Stability of Lenalidomide in Methanol/Ethanol Solvents Using RP-HPLC-UV and LC-MS
Lenalidomide is a heterocyclic drug used for the treatment of Myelodysplastic syndrome. The current research focuses on the structural elucidation of new degradants that are formed unexpectedly upon storage of lenalidom...
Evaluation of Basella alba L. Mucilage as a Suspending Agent in Metronidazole Suspension
In the quest for natural biodegradable, non-toxic polymers for use as excipients in pharmaceutical formulations, mucilage of Basella alba L (BAM) stem was isolated and evaluated as a suspending agent in metronidazole su...
Molecular modeling studies of some phytoligands from Ficus sycomorus fraction as potential inhibitors of cytochrome CYP6P3 enzyme of Anopheles coluzzii
The major obstacle in controlling malaria is the mosquito’s resistance to insecticides, including pyrethroids. The resistance is mainly due to the over-expression of detoxification enzymes such as cytochromes. Insectici...