Antifungal Properties of Ozone Gas in Stored Naturally Contaminated Dry Maize (Zea mays L.) Grains
Journal Title: Scholars Journal of Engineering and Technology - Year 2017, Vol 5, Issue 4
Abstract
Antifungal properties of ozone (O3) gas were evaluated in stored naturally contaminated dry maize grains. An experimental design was applied at three concentrations (20, 40, 60 μmol/mol) and different exposure times (30 to 180 min) for Groups I to III, respectively. O3 gas had evaluated its antifungal efficiency at Day zero and after 30 days of storage, at the lower and upper layers of each silo. Regarding O3 concentration, it was observed with the increase of its concentration (from 20 to 60 µmol/mol) a decrease of 2.5x10(16.2%) and 0.5x10(3.2%) CFU/g in the silo upper and the lower layers, respectively. On the other hand, regarding O3 time of exposure, when the ozonation time increased (from 30 to 180 min) on contaminant mycoflora there was a total fungi load decrease of 1.0x10(6.5%) and 0.5x10(3.2%) CFU/g in the upper and lower layers, respectively exposed to O3 gas. The response surface presented the maximum of 94.5% of spores inhibition. After 30 days of storage no statistical difference was observed between the applied treatments. Therefore, both treatments were effective. It was observed at Control silo (no gas treatment) at day 30th a total fungi load of 22x10 CFU/g. The O3 treated, at day 30th had in the upper layer 1.3x10 CFU/g and in lower layer 0.4x10 CFU/g, which represents 93.8% (max 97.7%) and 98.1% (max 100%, i.e., NG= no growth) of spores inactivation in the upper layer and lower layer, respectively. In this study, spores, can be efficiently destroyed by the O3 gas, with 88% inhibition of the spores immediately after application and 30 days after application, 100% of spores do not germinate under the conditions of 60 μmol/mol and 180 min. Keywords: Contamination; ozonation; maize; quality, fungi.
Authors and Affiliations
Christ D, Kreibich HH, Valmorbida R, Savi GD, Silva JR, Scussel VM
Keywords
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