ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND PANCREAS REGENERATION ACTIVITIES OF BLACK CUMIN (NIGELLA SATIVA L.) SEEDS ETHANOL EXTRACT IN ALLOXAN-INDUCED DIABETIC RATS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 5
Abstract
Objective: The goal of this study was to determine the antihyperglycemic, antioxidant as well as pancreas regeneration effects of ethanol extracts of Nigella sativa L. seeds in alloxan-induced diabetic rats.Methods: The male Wistar rats were induced by intraperitoneal injection of alloxan (150 mg/kg bw). N. sativa seeds extract was prepared and orally administrated at the dose of 125 and 250 mg/kg bw for 28 d. Blood glucose level was measured, and antioxidant status was determined by the activities of superoxide dismutase, glutathione peroxidase as well as malonyl aldehyde in liver. Histopathological and immunohistochemistry study of the pancreas was conducted at the end of the experimental period. The data obtained were analyzed by one-way ANOVA (p<0.05).Results: Both doses of N. sativa seeds extract showed the glucose lowering effect and corrected antioxidant status of diabetic animals in liver. N. sativa seeds extract dose of 250 mg/kg decreased blood glucose levels and malonyl aldehyde, as well as increased the activity of superoxide dismutase and glutathione peroxidase, more effective than a dose of 125 mg/kg. Immunohistopathology profiles proved the pancreas regeneration activity of N. sativa seeds extract, based on the enhanced of diameter and amount of Langerhans islet cells.Conclusion: N. sativa seeds extract dose of 125 and 250 mg/kg showed antihyperglycemic effect, enhanced antioxidant activity, as well as pancreas regeneration from organ damage on an alloxan-induced diabetic rat.Keywords: Nigella sativa L. seed extract, Antihyperglycemic, Antioxidant, Pancreas regeneration
Authors and Affiliations
Gunawan P. Widodo, Rina Herowati, Jason M. Perangin-angin, Jefri E. y. Kamlasi
DIRECT COST OF TREATMENT OF DIABETES MELLITUS TYPE 2 IN PAKISTAN
Objective: Diabetes is a serious illness. It is a key and growing threat to the health of the global world. The prime concern of this research was to estimate the cost-of-illness of type 2 diabetes in Karachi, a major me...
UV SPECTROPHOTOMETRIC DETERMINATION OF RUPATADINE FUMARATE IN BULK AND TABLET DOSAGE FORM BY USING SINGLE POINT STANDARDIZATION METHOD
Objective: To develop and validate a simple, selective, precise and accurate method for the estimation of rupatadine fumarate in bulk and tablet dosage form by using the single point standardization method as per interna...
THE THERAPEUTIC AND NEUROPROTECTIVE EFFECTS OF GREEN TEA IN A RAT MODEL OF TERLIPRESSIN-INDUCED CHRONIC HYPONATREMIA
Objective: Hyponatremia (HN) is associated with mortality and morbidity risks due to the development of hyponatremic encephalopathy. Its rapid correction also carries a high risk of development of the serious cerebral di...
IN SILICO STUDIES ON FUNCTIONALIZED AZAGLYCINE DERIVATIVES CONTAINING 2, 4-THIAZOLIDINEDIONE SCAFFOLD ON MULTIPLE TARGETS
Objective: The 2, 4-thiazolidinedione containing compounds could lead to most promising scaffolds with higher efficiency toward the targets recognized for its antidiabetic activity when combined with azaglycine moiety. T...
PROXIMATE COMPOSITION, NUTRITIVE VALUE AND EVALUATION OF ANTIOXIDANT POTENTIAL OF STEM OF DRACAENA REFLEXA LAM
Objective: The stem of Dracaena reflexa was analyzed for preliminary phytochemical screening, proximate composition, nutritive value, total phenolic content and antioxidant potential.Methods: Preliminary phytochemical sc...