Abstract

Objective: To investigate the effect of standardized methanol extract of Evolvulus alsinoids Linn. (E. alsinoids) on Plasmodium falciparum specific lactate dehydrogenase (PfLDH) enzyme inhibition. Methods: To carry out enzyme inhibition studies, lactate dehydrogenase was cloned from Plasmodium falciparum 3D7 strain using expression vector pET28a and expressed in Escherichia coli BL21 (DE3). Protein purification was carried out by Ni-affinity chromatography. This protein was used for the inhibition study. Methanol extract of E. alsinoids was standardized by high performance thin layer chromatography described by previous literature and screened for PfLDH enzyme inhibitory activity and compared with gossypol (a known PfLDH enzyme inhibitor). Results: It was found that E. alsinoids possesses a compond scopoletin and potentially inhibits PfLDH [(25.04依0.51)%]. Conclusions: Methanol extract of E. alsinoids showed significant PfLDH inhibition as evidenced from the experiments performed. The activity may be attributed to the presence of various polyphenolic and flavanoids compounds.

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