ANTIMICROBIAL ACTION OF LED RADIATION ON CAUSATIVE AGENTS OF OPPORTUNISTIC INFECTIONS
Journal Title: Вісник Одеського національного університету. Біологія - Year 2018, Vol 23, Issue 1
Abstract
The antibiotic resistance of microorganisms-causative agents of opportunistic infections significantly reduces the effectiveness of treatment of diseases caused by Pseudomonas aeruginosa and Candida albicans. The aim of the study was to investigate the direct influence of LED radiation of different wavelengths on the growth rate of P. aeruginosa and C. albicans on solid nutrient media. Materials and methods. Pure cultures of the collection strains of P. aeruginosa ATCC 27853 and C. albicans ATCC 90028 were used for the studies. Irradiation of standardized cultures was carried out with LED radiation of red-infrared and blue-infrared ranges. The results were measured by counting the number of microorganisms’ colonies after 24-hours incubation in a thermostat at 37 °C and compared with the control – unirradiated cultures. Studying the effect of radiation of different wavelengths, exposures and frequencies has been done by separate series. Results of the research. It is shown that LED radiation has a pronounced effect on the growth rate of the studied microflora. The effect of influence directly depends on the exposure to the irradiation: 5-10-minute irradiation stimulates the growth of microbes, whereas 20-25-minute exposures have a pronounced bactericidal (fungicidal) effect. In addition to the exposure, the frequency of LED radiation has a significant effect on the intensity of growth – the most significant inhibitory effect was provided by radiation with frequency of 8000 Hz. Conclusions. In case of direct exposure of the studied collection strains of P. aeruginosa ATCC 27853 and C. albicans ATCC 90028 LED radiation of red-infrared and blue-infrared ranges produces a photomodifying effect on the intensity of their growth. With the optimum radiation parameters set – exposure 20 minutes, frequency 8000 Hz – the number of colonies P. aeruginosa and C. albicans decreased, respectively, by 79,1-79,3 % and 25,8-48.2%.
Authors and Affiliations
V. V. Pantyo, V. I. Pantyo, E. M. Danko
Keywords
Related Articles
- EP ID EP502848
- DOI 10.18524/2077-1746.2018.1(42).118457
- Views 63
- Downloads 0
VEGETATIVE SUPPORT OF AUDITORY-MOTOR CHOICE REACTION IN BOYS WITH VISUAL DYSFUNCTIONS
Introduction. Visual dysfunctions may cause to changes in the autonomic regulation of the heart rate due chronic social and emotional stress and because of direct influence of reduction visual inputs on higher autonomic...
ANTIOXIDANT STATUS OF RAPANA TISSUES UNDER STRESS CAUSED BY COPPER IONS
Rapana venosa is widely spread in the world's oceans and has adapted well to the conditions of different pollution. The study of adaptation options of Rapana was the purpose of this work. We studied the antioxidant syste...
PHYSIOLOGICAL INDICATORS AND YIELD OF GRAPE VARIETIES AROMATNIY AND CABERNET SAUVIGNON TREATED WITH DRUG AGROMAR
Introduction. Drug AgroMar based on Trichoderma lignorum H. is an ecologically safe biofungicide whose effectiveness in cultivating technical varieties of grapes has not been sufficiently studied under conditions of the...
PHYSIOLOGICAL ACTIVITY OF SHIZOPHYLLUM COMMUNE FR.: FR. AND TRAMETES VERSICOLOR (L.: FR.) QUEL. CULTIVATED ON BREADCRUMB IN SUBMERGED CULTURE
Shizophyllum commune and Trametes versicolor are well-known medicinal fungi which belonged to phylum Basidiomycota and possess immunomodulating, antimicrobial and anticancer activity. The aim of current study was to inve...
MUCOSA-ADHESIVE GELS WITH QUERCETIN – EFFECTIVE MEDICINAL FORM FOR CORRECTION OF METABOLIC DISTURBANCES
Introduction Mucosaadhesive gels are applied to mucous membranes. Advantages: 1) presence of the active substance on the mucosal surface is prolonged; 2) it is possible to increase the concentration of active ingredient...