Antimicrobial Activity and Antibiotic Sensitivity of Lactic Acid Bacteria Isolated from Fermented Foods and a Commercial Probiotic: A Comparative in Vitro Study
Journal Title: International Journal of Environment, Ecology, Family and Urban Studies (IJEEFUS) - Year 2019, Vol 9, Issue 4
Abstract
Five strains of Lactic acid bacteria isolated from fermented foods in the previous study,identified as L. lactis BS14-17, L. lactis YS8-4, L. lactis subsp. cremorisst YS 8-4,Bifidobacterium bifidum NCTC 13001, Bifidobacterium lemurum, labeledas(LAB1, LAB2, LAB3, BIF1,BIF2) & a commercial probiotic Lactococcus lactis (PBIO) were evaluated for their degree of antagonism towards selected human pathogens & food spoilage causing bacteria such as S. aureus, B. subtilis, S. typhimurium, B. cereus, E. coliusing agar well diffusion assay. Three isolates (LAB1, LAB2, LAB3)& the commercial probiotic (PBIO)were studied for their response towards twelve antibiotics by disc diffusion assay. Results were compared by measuring the zones of inhibition for evaluating microbial antagonism & antibiotic sensitivity profiles as well as graphically represented. All five LAB isolates showed microbial antagonism against selected pathogens. Antibacterial activity of LAB isolates varied & ranged between 5-16 mm. On an average LAB isolate BIF1 showed the highest degree of antagonism towards all pathogens. BIF1 showed highest zone of inhibition (16mm) against B. subtilis. Isolates LAB3 & BIF 2 showed the least degree of antagonism. The degree of antibacterial property of isolates & commercial probiotic was in the decreasing order as BIF1 > LAB1> PBIO > LAB2 >BIF2 >LAB3. As such two isolates BIF1 and LAB1 showed more antagonism towards pathogens when compared to commercial probiotic (PBIO). However commercial probiotic showed highest antagonism (ZOI=14mm) towards S. aureus than all LAB isolates in this study. The three isolates of LAB (LAB1, LAB2, LAB3) as well as commercial probiotic (PBIO) were further studied for their antibiotic sensitivity using twelve commercial antibiotic discs. Almost all showed sensitivity towards most of the antibiotics.
Authors and Affiliations
Baba Shamsuddin, Mohammed Mazharuddin Khan
Keywords
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- EP ID EP651930
- DOI 10.24247/ijeefusaug20197
- Views 107
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