Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 1
Abstract
Objective: To assess the probiotic nature of Lactobacillus in preventing cervical pathogens by studying the effectiveness of antimicrobial activity against vaginal pathogens. Methods: Lactobacilli were isolated from healthy vaginal swabs on selective media and different pathogenic bacteria were isolated by using different selective media. The Lactobacillus strains were tested for the production of hydrogen peroxide and antimicrobial compounds along with probiotic properties. Results: Of the 10 isolated Lactobacillus strains, strain 1, 3 and 6 are high hydrogen peroxide producers and the rest were low producers. Results of pH and amines tests indicated that pH increased with fishy odour in the vaginal fluids of cervicovaginal infection patients when compared with vaginal fluids of healthy persons. The isolates were found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsule forming and catalase-negative bacilli. The results of antimicrobial activity of compounds indicated that 280 and 140 µg/mL was the minimum concentration to inhibit the growth of both pathogens and test organisms respectively. Conclusions: The results demonstrated that Lactobacillus producing antimicrobial compounds inhibits the growth of cervical pathogens, revealing that the hypothesis of preventing vaginal infection by administering probiotic organisms has a great appeal to patients, which colonize the vagina to help, restore and maintain healthy vagina.
Assessment of gastroprotective effect of crude tannin from Schwenkia americana Linn. on mitochondrial functions in ulcerogenic rats
Objective: To investigate the gastroprotective effect of tannin from Schwenkia americana Linn. on mitochondrial functions in ulcerogenic rats. Methods: A total number of 36 male Wistar rats weighing 160–220 g were used f...
Biological synthesis of silver nanoparticles by using Viola serpens extract
Objective: To formulate a biological approach for the biological synthesis of silver nanoparticles using aqueous extracts of leaves of Viola serpens which is considered as a ecofriendly method as it does not include any...
Community knowledge and perceptions on the management of non-malarial fevers under reduced malaria burden and implications on the current malaria treatment policy in Morogoro, Tanzania
Objective: To investigate community knowledge and perceptions on the management of nonmalarial fevers under reduced malaria burden and the implications on the uptake of artmetherlumefantrine (ALu) for malaria treatment....
Co-infection of HIV and HBV in voluntary counseling and testing center in Abidjan
Objective: To evaluate the co-infection of hepatitis B virus (HBV) and immune deficiency virus (HIV) among clients consulting at the Voluntary Counseling and Testing Center (VCT Center) of the Institut Pasteur de C ôte d...
Phytochemical evaluation and molecular characterization of some important medicinal plants
Objective: Phytochemical evaluation and molecular characterization of plants is an important task in medicinal botany and drug discovery. In the current study, Ocimum species, Pimenta officinalis and Piper betel were con...