ANTIMICROBIAL ACTIVITY OF PHOTODYNAMIC THERAPY AGAINST MICROORGANISMS ISOLATED FROM DEEP CARIOUS LESIONS.

Journal Title: Journal of IMAB - Annual Proceeding (Scientific Papers) - Year 2013, Vol 19, Issue 4

Abstract

Introduction: Photodynamic therapy (PDT) is an innovative method, supporting routine treatment methods and accelerating the healing process. It is a method for treatment of pathogens and tumor cells with photoactive dye, in the presence of oxygen. PDT is minimally invasive and high- selective method against specific cells and is equally effective against sensitive and resistant to antibiotics microorganisms. Aim: The purpose of this study was to determine the antimicrobial activity of the PDT (Fotosan-Agent High) against microorganisms isolated from deep carious lesions. Materials and methods: For the purpose of this study were selected 20 patients ( n=20) with primary, deep carious lesions on permanent teeth with complete root development All prepared cavities were class I and class II, and the treatment method was indirect pulp capping. Cavities were prepared with high and low-speed handpieces and water- air cooling. Two microbiological samples were taken of each cavity: Group 1 - Microbiological samples after cavity treatment with Aqua destillata (n=20) Group 2 - Microbiological samples after cavity treatment with PDT (Photosensitizer Fotosan (Agent high) and irradiation with a diode laser, λ = 632 nm, for 60 seconds)(n=20) The samples were placed in eppendorf tube with standard transport medium and transported to the microbiological laboratory for determination of microbial number and identification of the isolated microorganisms. The statistical analysis was made with non- parametric test of` McNemar. Results: In 82.35% of cases (14 of 17 cases) after treatment bacteriаl infection was totally eliminated. In 17.65% (3 of 17 cases) was determined reduction of bacteria count from 100 to 1000 times. The statistical analysis with non- parametric test of` McNemar presented significantly higher proportion of cases with bacterial elimination after photodynamic therapy ( p < 0.001). Conclusion: After photodynamic desinfection were determined considerable elimination of microbial infection and reduction of bacterial variety and bacterial number. On the ground of these results it can be concluded, that photodynamic therapy is an effective method of treatment and removal of microorganisms from deep carious lesions and support the healing processes in the dentin and dental pulp.

Authors and Affiliations

Violeta Dogandzhiyska, Raina Gergova, Slavcho Dimitrov, Maya Doychinova

Keywords

Related Articles

ABDUCENS NERVE PALSY AND THROMBOSIS OF THE CEREBRAL VEINS AND SINUSES - A DIAGNOSTIC PITFALL

Thrombosis of the cerebral veins and sinuses is an infrequent cerebrovascular disorder. Because the highly variable symptoms, recent neuroimaging plays a key role in the diagnosis. Abducens nerve palsy as a focal neurolo...

 COMPARATIVE ANALYSIS OF MEDICAL EQUIPMENT USED IN DIAGNOSTIC IMAGING IN BULGARIA AND COUNTRIES WORLDWIDE AND OPTIMIZATION AIMED AT IMPROVING THE HEALTH CARE SYSTEM MANAGEMENT

 Purpose: to reveal the current condition of medical equipment in Bulgaria related to those major groups of socially significant diseases and to make an attempt to define guidelines for its optimization in view of i...

“ERGONOMIZATION” OF THE WORKING ENVIRONMENT AND BUILDING UP OF HEALTHY WORKING POSTURE OF DENTAL STUDENTS

The purpose was to assess the knowledge of dental students of the ergonomics’ principles and how they develop adequate skills to work safe in clinic. Methods: A questionnaire, comprising a set of 25 close-ended questions...

THE DYNAMICS OF ANTIMICROBIAL RESISTANCE OF Salmonella typhimurium ISOLATES

Salmonella typhimurium is the most common pathogen isolated in foodstuffs toxin infections. The development of antibiotic resistance in Salmonella typhimurium over the last twenty years is caused by an extensive applicat...

SURGICAL TREATMENT AND RECONSTRUCTION FOR CENTRAL GIANT CELL GRANULOMA OF MANDIBLE

Introduction: Central giant cell granuloma (CGCG) is a benign aggressive destructive osteolytic lesion of osteoclastic origin. The central giant cell granuloma is often found in the mandible, anterior to the first molars...

Download PDF file
  • EP ID EP151944
  • DOI 10.5272/jimab.2013194.430
  • Views 83
  • Downloads 0

How To Cite

Violeta Dogandzhiyska, Raina Gergova, Slavcho Dimitrov, Maya Doychinova (2013). ANTIMICROBIAL ACTIVITY OF PHOTODYNAMIC THERAPY AGAINST MICROORGANISMS ISOLATED FROM DEEP CARIOUS LESIONS.. Journal of IMAB - Annual Proceeding (Scientific Papers), 19(4), 430-434. https://europub.co.uk/articles/-A-151944