Antimicrobial Activity of Potato Rhizospheric Pseudomonas chlororaphis subsp. aureofaciens from Sétif Algeria
Journal Title: Annual Research & Review in Biology - Year 2016, Vol 11, Issue 5
Abstract
Aims: This study was assessed to demonstrate the antimicrobial activity in vitro of an identified fluorescent Pseudomonas strain characterized for its capacity to produce phenazine compounds. Methodology: First Pseudomonas chlororaphis subsp aureofaciens was inoculated on Nutrient Broth supplemented with Yeast Extract (NBY) and with glucose at a final concentration of 2%, after incubation the filtered culture was acidified with HCl to pH 2. The solution was extracted twice with the same volume of ethyl-acetate. The organic supernatants were combined, dried over anhydrous Na2SO4, and evaporated to dryness. The crude extract was resuspended in methanol and tested for antimicrobial activity. Antimicrobial activity was determined (i) by disc diffusion technique for bacteria and (ii) using serial dilution technique in soft PDA for fungi. Secondly the antifungal activity of the bacterial strain was tested against several phytopathogenic fungi in dual culture. Results: The studied strain has an important activity against the phytopathogenic bacteria and fungi tested. Among the tested fungi Fusarium oxysporum f. sp. albedinis is the most sensitive to the actions of this Pseudomonas, where the inhibition rate reached 77.78%. The less sensitive one was Pythium ultimum with a rate of 55.56%. While for pathogenic bacteria only Salmonella enteridis was sensitive to the tested strain. Conclusion: Pseudomonas chlororaphis subsp aureofaciens showed appreciable antagonistic activity, in vitro, against special forms of Fusarium oxysporum and the tested phytopathogenic bacteria.
Authors and Affiliations
Samia Mezaache-Aichour, Nora Haichour, Jane Nicklin, Mohamed M. Zerroug
Keywords
Related Articles
- EP ID EP350976
- DOI 10.9734/ARRB/2016/31651
- Views 83
- Downloads 0
Effects of Aqueous Extract of Allium sativum on Biochemical Parameters and Oxidative Stress in STZ- and STZ+niacinamide-induced Diabetes Mellitus Rats
Background: Diabetes mellitus (DM) is a common metabolic disorder with disruption of glucose and lipid metabolism. Many herbal extracts are used for treatment of diabetes. Allium sativum, (garlic), is one of a plant that...
Roosting Behaviour of Black-winged Kite (Elanus caeruleus) Inhabiting the Arid Zone of Rajasthan
The word “Roost” means “a sleeping house of fowls”. The roosting ecology of Black-winged kite was studied from January 2012 to December 2014 in rural and urban areas of Churu city and rural areas of Ratangarh of Churu di...
Genetic Diversity and Distribution of Cassava Brown Streak Virus and Ugandan Cassava Brown Streak Virus in Major Cassava-growing Regions in Kenya
Cassava brown streak disease (CBSD) is caused by two viruses; Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) that are genetically distinct. The disease can cause losses of up to 100 mill...
All-Trans Retinoic Acid Downregulates Epidermal Growth Factor Receptor Mediated Regulation of Matrix Metalloproteinase-2 in B16F10 Murine Melanoma Cells
Aims and Study Design: Epidermal growth factor receptor (EGFR) regulates a number of cellular processes, including cell motility, proliferation, differentiation and survival. EGFR mediated signal transduction via mitogen...
Cognitive Function in Male Track and Field Iranian National Team Athletes
Background: Cognitive function including decision making and short term memory are affected by environmental factors including physical activity. Data showed that sustained physical activity can improve learning and memo...