Antimicrobial Activity of the Leaf and Seed Extracts of Moringa Oleifera on Some Bacteria Isolates
Journal Title: Journal of Medical Science And clinical Research - Year 2015, Vol 3, Issue 1
Abstract
This study focused on the antimicrobial activity of alcohol (ethanol) and aqueous extracts of Moringa olifera seed and leaf on Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The ethanol and aqueous extracts of the seed and leaf at concentration of 10g/100ml and the diluted ethanol and aqueous extracts at concentration of 0.1g/ml, 0.2g/ml, 0.4g/ml, 0.6g/ml and 0.8g/ml were impregnated into sterilized filter paper discs. Also, ethanol alone was impregnated into sterilized filter paper discs and use as control. Agar disc diffusion method was adopted to obtain zones of inhibition. The zones of inhibition obtained for ethanol extract of Moringa olifera seed for Staphylococcus aureus was 12mm, Escherichia coli was 13mm and Pseudomonas aerucginora was 10mm. The ethanol extract of Moringa olifera leaf gave an inhibitory zone of 11mm for Staphylococcus aureus, 10mm for Escherichia coli, 9mm for Pseudomonas aeniginosa .Different degrees of inhibition was observed for the diluted ethanol extracts of Moringa olifera seed. The diluted ethanol extracts of Moringa olifera leaf were resistant to the test organisms at concentration of 0.1g/ml, 0.6g/ml but was sensitive at concentration 0.8g/ml. The aqueous and diluted aqueous extracts of Moringa olifera seed and leaf were resistant to the test organisms. The zones of inhibition obtained for ethanol alone on the test organisms ranged between 8mm – 10mm. The degree of zone of inhibition differs, however the ethanol extracts exhibited the highest inhibitory effect than the aqueous extracts. This study revealed that Moringa olifera seed and leaf extracts are potential antimicrobial agents.
Authors and Affiliations
Mbata Christian A
Keywords
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