Antimicrobial and radical scavenging activities of Moroccan Ziziphus lotus L. seeds
Journal Title: The Journal of Phytopharmacology - Year 2019, Vol 8, Issue 4
Abstract
Natural plant extracts contain a variety of phenolic contents, which are associated to various biological activities. In this study, we evaluated the antioxidant and antimicrobial activities of organic (ethanol and methanol) and aqueous extracts prepared from Ziziphus lotus L seeds. The total polyphenol content of the extracts was determined using the Folin-Ciocalteu reagent, it was in the range of 50.67 ± 1.44 (Ethanolic Extract), 39.32 ± 1.44 (Methanolic Extract) and 23.54 ± 0,44 (Aqueous Extract) mg gallic acid equivalent/g DW. The content in flavonoids was estimated at 69.19 ± 0.10 (Ethanolic Extract), 53.13 ± 0.55 (Methanolic Extract) and 9.63 ± 0.88 (Aqueous Extract) mg equivalent quercitin/g DW. The condensed tannin assay revealed that the methanol extract was rich on tannin (9.12 ± 1.07 mg/g) relatively to the ethanol and aqueous extracts (4.97 ± 0.95 and 1, 88 ± 0.47 mg/g respectively). The antioxidant activity was evaluated in vitro by DPPH and phosphomolydbate (total antioxidant capacity). The results reveal that the three extracts have a capacity to trap the DPPH radical with IC50 1.33 ± 0.01, 1.32 ± 0.09 and 3.11 ± 0.05 mg/ml for the methanol, ethanol and aqueous extract respectively. This antioxidant activity is confirmed by the phosphomolybdate test. The antimicrobial activity of the studied extracts was evaluated using the broth microdilution, on five microbial strains: Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis and Candida tropicalis. Results revealed an activity on the four bacterial strains tested. While the fungal strain (Candida tropicalis) showed an amount of resistance to the tested extracts. All this results could justify the use of Ziziphus lotus in the treatment of various infections in traditional environments.
Authors and Affiliations
Rais Chaimae, Benidir Meryem, Slimani Chaimae, EL-Ouazna Bouchamma, Ettadili Hamza, ElHanafi Laila, EL Ghadraoui Lahsen, Benjelloun Meryem
Keywords
Related Articles
- EP ID EP49940
- DOI http://www.phytopharmajournal.com/Volume8issue4.html#
- Views 190
- Downloads 0
Phytochemical, Nutritional and Pharmacological evidences for Abelmoschus esculentus (L.)
Okra is a nutritional source of power used throughout history for both medicinal and culinary purposes. Okra is good source of minerals, vitamins and nutrients that are responsible for the health benefits. It has variou...
Phytopharmacology of Alternanthera brasiliana (Amaranthaceae): A Brazilin plant
Alternanthera brasiliana L. (Amaranthaceae) is evergreen, perennial herbs, native to tropical and sub-tropical regions of Brazil, Australia & South America. Alternanthera are grown for edging beds and for bedding. The...
Role of Dhoopan Karma and Gauradya Ghrita in the management of Dushta Vrana (infected wound)
Ayurveda is that which deals with good, bad, happy and unhappy life, its promoters and non-promoters, measurement and nature. Shalya Tantra is a specialized branch of Ayurveda in which Surgical, Medicinal and Parasurgi...
GC-MS and FT-IR Profiling of leaves methanol extract from the Pleiospermium alatum (Wall. ex Wt. & Arn) Swingle Rutaceae family
The present study was aimed to analysis of bioactive constituents of leaves from the Pleiospermium alatum (Rutaceae). The methanol extract of the leaves were subjected to Fourier transform infrared spectroscopy (FTIR) a...
Hepatoprotective potentials of Picralima nitida against in vivo carbon tetrachloride-mediated hepatotoxicity
This research aimed at investigating the in vivo Carbon tetrachloride (CCl4)-mediated hepatotoxicity of methanolic seed extract of Picralima nitida (P. nitida) using Wistar rats. Twenty five (25) rats randomly selected...