Antimicrobial Effect of Different Root Canal Medicaments on Enterococcus Faecalis: in vitro Comparative Study
Journal Title: International Journal of Dentistry and Oral Science (IJDOS) - Year 2014, Vol 1, Issue 2
Abstract
Introduction: Enterococcus faecalis (E. faecalis) is a microorganism commonly detected in persistent endodontic infections. Eradication of this organism from an infected root canal remains a significant clinical challenge for modern endodontic treatment. So, this in vitro study was conducted to compare antimicrobial effect of some root canal medicaments on E. faecalis. Materials/Methods: A total of 50 E. faecalis strains (49 strains were selected randomly from positive cultures together with a standard E. faecalis strain ATCC 29212) were used in the study. Inoculum for each bacterial strain was prepared in a turbidity of 0.5 on McFarland scale. The prepared discs of the three intracanal medicaments; chlorhexidine, diapex and diclofenac; together with readymade discs of amoxicillin and doxycycline were distributed on blood agar and Mueller-Hinton agar plates inoculated with an E. faecalis suspension previously prepared. Antimicrobial activity was assessed by measuring the diameters of the zones of growth inhibition surround the discs after incubation at 37°C for 24 hours and then after 48 hours. Results: All strains (100%) were sensitive to chlorhexidine even though strains that showed resistance to one or both of antibiotic used (amoxicillin, doxycycline). On the other hand, all strains (100%) were resistant to diapex and diclofenac even standard strain. Sixty eight percent of the tested strains were sensitive to doxycyline and 44% of them were sensitive to amoxicillin. Conclusion: Under the conditions of this study, chlorhexidine is very effective against E. faecalis and can be considered as the most powerful root canal medicament compared to other intracanal medicaments tested. Controversially, no zones of inhibition were detected around diapex and diclofenac.
Authors and Affiliations
Ibtesam K Afifi
Keywords
Related Articles
- EP ID EP194848
- DOI 10.19070/2377-8075-140004
- Views 255
- Downloads 0
The Importance of Methods Imaging in the Diagnosis of Displacement to Mandibular Molar Root Fragment: Case Report
The aim of this study discusses the causes, prevention measures, diagnostic means for locating, treatment the displacement of the lower third molar root to the submandibular space during extraction. This article describe...
Cytotoxic Effect of Chitosan-H, Resveratrol, β-Carotene and Propolis and their Chitosan Hydrogels on Balb/C Mouse 3T3 Fibroblast Cells
Background/purposes: The beneficial effect (bond strength and longevity) of the addition of different chitosan/antioxidant hydrogels to dental restoratives was reported. However, it still needs to be verified whether the...
Ketofol as a Balanced Anesthetic for Procedural Sedation and Analgesia (PSA) in the Obese Oral Surgery Patient: a Commentary
A hallmark of oral and maxillofacial surgery is the administration of sedation and general anesthesia in the office setting. As part of their training, oral surgeons are required to function as anesthesia residents for a...
Neurofibromatosis type I with an Rare Oral Manifestation
Neurofibromatosis type I is one of the most common genetic diseases and it may have oral manifestation. Objective: To report a case about a 33 years old female patient diagnosed with neurofibromatosis type I in the skin...
Respect of the Canal Path: Comparative Study between WaveOne and Protaper Universal
Aim: This study compares two major parameters regarding the respect of the canal path during the shaping: the canal deviation and the centering ability between two different systems on human canal teeth by using cone bea...