Antimicrobial photodynamic therapy on Candida albicans on acrylic surfaces of dental prostheses. In vitro study
Journal Title: Revista Eugenio Espejo - Year 2022, Vol 16, Issue 3
Abstract
Species of the genus Candida are frequently found in the oral microbiota of humans. Objective: to compare the antimicrobial effectiveness of photodynamic therapy on Candida albicansstrains on acrylic surfaces for dental prostheses by using a 660nm wavelength laser and methylene blue as a photosensitizing agent, with respect to other therapeutic methods. Methodology: in vitro study, using 60 thermosetting acrylic discs immersed in a suspension of C. albicans, generating a biofilm simulation on the surface of a dental prosthesis. After that, five random groups of 12 units were formed to apply them different therapeutic procedures: G1 (saline solution), G2 (chlorhexidine 0.12%), G3 (nystatin in topical solution at 0.001ml/ul), G4 (0.005% methylene blue + diode laser with λ=660nm, 100mW, 32J/cm2) and G5 (0.01% methylene blue + diode laser with λ=660nm, 100mW, 321J/cm2). Results: a statistically significant difference was observed when comparing groups G4 and G5 with respect to G1 and G3 (p<0.05). Conclusion: the efficacy in reducing the number of viable C. albicans CFU was higher in the groups where laser radiation with a wavelength of 660nm was used with different concentrations of methylene blue. The use of nystatin and physiological saline had the lowest efficacy values.
Authors and Affiliations
María Alejandra Jarrín Ríos, Maria Fernanda Caicedo Breeddy, Pablo Ruben Garrido Villavicencio, Héctor Eduardo Cepeda Inca
Keywords
Related Articles
- EP ID EP708782
- DOI https://doi.org/10.37135/ee.04.15.08
- Views 44
- Downloads 0
Enfermería en la atención multidisciplinaria de la hipoacusia neonatal
The adequate psychomotor development of the child is determined by different physiological processes. Taking into consideration what is mentioned above an observational, descriptive, cross-sectional, and mixed study was...
Características de la maternidad adolescente en un hospital del segundo nivel del contexto ecuatoriano
The vulnerability of adolescents is accentuated during motherhood due to possible biopsychosocial complications for the mother-child binomial. An observational, descriptive, and cross-sectional study was developed with a...
Characterization of the state of general mental health in professional drivers of the central Ecua-dorian highlands
A descriptive, cross-sectional and correlational study was carried out in order to characterize the state of mental health in a group of individuals from the central Ecuadorian Highlands. The studied variables were somat...
Hipoclorito de sodio al 5% Vs digluconato de clorhexidina. Desinfectantes antimicrobianos del sistema de irrigación odontológico
A comparative observational study was carried out in the Dental Clinic of Universidad Nacional de Chimborazo (UNACH), during the academic period October 2015 - February 2016. The object of study was given by the existing...
Socioeconomic factors and academic performance in nursing students. Integrative literature review
The successful training of nursing professionals is a necessity for contemporary society. Thus, this research aimed to establish the regularities reported by different authors about the way in which socioeconomic factors...