Antimicrobial Resistance of Klebsiella pneumoniae -ESBL Producing Strains Isolated from Clinical Specimens in Abidjan (Cote de Ivoire)
Journal Title: Microbiology Research Journal International - Year 2017, Vol 20, Issue 2
Abstract
Aims: The objective of this study is to detect the prevalence of qnr genes in broad-spectrum beta-lactamase producing Klebsiella pneumoniae, involved in hospital and community-acquired infections. Study Design: It is a retrospective study. Place and Duration of Study: Bacteriology and virology laboratorie of Institut Pasteur, Abidjan, Côte d'Ivoire. Methodology: From January 2011 to June 2016, 350 Klebsiella pneumoniae were isolated from various clinical specimens and identified by conventional bacteriological tests. Antibiotic resistance (beta-lactams, quinolones and aminoglycosides) and detection of broad-spectrum beta-lactamases were assessed by the diffusion method and the synergy test, respectively. Molecular characterization of quinolone resistance genes (qnr A, qnr B and qnr S) was performed by the conventional polymerase chain reaction (PCR). Results: Of the 350 Klebsiella pneumoniae isolates, 91(26%) were detected as ESBL producer, 36.2% (n=33) recovered from urine, 24.2% (n=22) from pus and 20.8% (n=19) from blood culture respectively. 46(33%) strains were idenifed to carry qnr genes, qnrB predominate 33(71%), followed by qnr 12(26.1%) and qnrA 1(2.2%). The strains exhibited high resistance to most of the agents tested, expect imipemen, low resistance to amikacin 4.1%, moderate to cefoxitin 31.8% and 54.9% with amoxicillin-clauvanic acid. Conclusion: Although these observed prevalences are small proportions, this can be seen as a warning signal for the future. The emergence and dissemination of resistance genes in Côte d'Ivoire could pose a public health problem. Thus, the establishment of a relevant resistance surveillance policy to better control the circulation of multidrug-resistant strains is necessary.
Authors and Affiliations
Joel Eric Tahou, Nathalie Kouadio Guessennd, Paulin Didier Sokouri, Valérie Gbonon, Fernique Konan, Jules Kouadio, Karine Kossia Gba, Baguy Mohamed Ouattara, Simon-Pierre Assanvo N’guetta
Keywords
Related Articles
- EP ID EP316033
- DOI 10.9734/MRJI/2017/34085
- Views 91
- Downloads 0
Exploring Textile Dye from Microorganisms, an Eco-friendly Alternative
Aims: Being an ancient art, the concept of dyeing using natural resource is neither a noble issue. To develop a green and sustainable world, the natural resources are now common sought. In this study screening of pigment...
Microbial Response to Varying Concentrations of Crude Oil Pollution of Agricultural Soils in Ondo State, Nigeria
This research investigated the effects of varying concentrations of crude oil on the population of crude oil degrading microorganisms in crude oil polluted agricultural soils from Igodan- Lisa, Oba-Ile and Ido-Ani areas...
Effects of Water Activity on the Radial Growth of Aspergillus niger on Solid Medium
The effects of water activity (aw) on the radial growth of Aspergillus niger on Malt Extract Agar was investigated using sodium chloride and glycerol mixtures. Varied concentrations of each solute was used to modify the...
Antifungal Resistance Patterns, Virulence Attributes and Spectrum of Oral Candida Species in Patients with Periodontal Disease
Aims: To observe the antifungal resistance pattern, virulence attributes and spectrum of Candida species in oral cavities of patients with periodontal diseases and healthy individuals. Study Design: A total number of 52...
Screening, Identification and Antibiotic Susceptibility Pattern of Bacteriocin-producing Lactic Acid Bacteria Isolated from Selected Traditionally Fermented Products
Aims: To isolate and identify bacteriocin-producing lactic acid bacteria from traditionally fermented products and determine their antibiotic susceptibility pattern. Place and Duration of Study: Department of Microbiolog...