Antimicrobial Susceptibility Profiling of Staphylococcus aureus Isolates from Milk
Journal Title: Microbiology Research Journal International - Year 2016, Vol 13, Issue 3
Abstract
Aims: This study investigated the occurrence of Staphylococcus aureus in milk and its sensitivity to twenty antibiotics. Study Design: The research was laboratory-based investigation. Place and Duration of the Study: The study was carried out at the Laboratory of Hygiene and animal pathology, University of Tiaret, Algeria, between September 2012 and May 2013. Methodology: Thirty eight milk specimens were collected from cattle and examined to estimate the prevalence of Staphylococcus aureus. The sensitivity of the isolates to twenty (20) antibiotics was evaluated and the presence of methicillin resistant Staphylococcus aureus (MRSA) was also determined. S. aureus was characterized using standard microbiological methods and confirmation was done using the API Staph Identification System. Antibiotic sensitivity of isolates was evaluated by means of agar diffusion technique while the minimum inhibitory concentration (MIC) was established using broth dilution technique for oxacillin, E-test for tetracyclin, and chloramphenicol respectively. Results: 55.26% of analyzed samples were contaminated with S. aureus. 100% of Methicillin Sensitive S. aureus were resistant to nalidixic acid, 70% to bacitracin, 65% to spiramycin, and 45% to penicillin and fosfomycin. There was no resistance to vancomycin, chloramphenicol, gentamicin and pristamycin among isolates. A total of 76% of the isolated strains were found to be resistant to at least 4 antibiotics. One Methicillin Resistant S. aureus strain (4.76%) was detected and showed multiple drug resistance. This resistance was crossed with all beta lactams and its resistance profile to macrolides was constitutive (MLSB const) while aminoglycosides phenotype was ANT (4’) (4’’). Conclusion: A high prevalence of S. aureus with multiple drug resistance was established. Improved food safety measures are thus necessary to prevent transmission and spread of antimicrobial resistance by these pathogens.
Authors and Affiliations
W. Chaalal, H. Aggad, K. Zidane, N. Saidi, M. Kihal
Keywords
Related Articles
- EP ID EP350844
- DOI 10.9734/BMRJ/2016/24064
- Views 101
- Downloads 0
Antimicrobial Activity of Moringa oleifera Leaf against Isolates of Beef Offal
This study was designed to evaluate the antimicrobial potentials of ethanol and aqueous extracts of Moringa oleifera against microorganisms isolated from beef offal. The beef offal samples (liver, kidney and intestine) w...
Hepatitis C Virus Genetic Diversity and Drug Mutational Analysis in Cameroon: 1992 to 2013
HCV infection is endemic in Cameroon. A Direct acting agent (DAAs), sofosbuvir that target the HCV non-structural 5B RNA-dependent RNA polymerase (NS5B RdRp) has recently been incorporated into the standard of care in Ca...
Detection of Carbapenem Resistance in Salmonella Species from a Tertiary Hospital in Eastern Cape, South Africa
Aims: Broad-spectrum carbapenem group is the current therapy for strains of Enterobacteriaceae that express extended spectrum beta-lactamases (ESBLs). However, recent reports of therapeutic failures of carbapenems with s...
The Diagnosis of Salmonella typhi Co-infection from Blood Samples of Clinically Suspected Typhoid Fever Patients at Some Hospitals in Ondo State, Nigeria
Background: Blood samples of clinically suspected typhoid fever patients were screened for bacterial and fungal co-infection. Methodology: The study was conducted in three different hospitals in Ondo State, Nigeria viz;...
Antibacterial Activity of Actinomycetes Isolated from Waste Dump Soil from Western Uganda
Aims: To determine the antibacterial activity of actinomycetes isolated from waste dump soil in Western Uganda. Study Design: The study was an experimental laboratory design. Place and Duration of Study: Waste dump soil...