Antimicrobial Susceptibility Profiling of Staphylococcus aureus Isolates from Milk
Journal Title: Microbiology Research Journal International - Year 2016, Vol 13, Issue 3
Abstract
Aims: This study investigated the occurrence of Staphylococcus aureus in milk and its sensitivity to twenty antibiotics. Study Design: The research was laboratory-based investigation. Place and Duration of the Study: The study was carried out at the Laboratory of Hygiene and animal pathology, University of Tiaret, Algeria, between September 2012 and May 2013. Methodology: Thirty eight milk specimens were collected from cattle and examined to estimate the prevalence of Staphylococcus aureus. The sensitivity of the isolates to twenty (20) antibiotics was evaluated and the presence of methicillin resistant Staphylococcus aureus (MRSA) was also determined. S. aureus was characterized using standard microbiological methods and confirmation was done using the API Staph Identification System. Antibiotic sensitivity of isolates was evaluated by means of agar diffusion technique while the minimum inhibitory concentration (MIC) was established using broth dilution technique for oxacillin, E-test for tetracyclin, and chloramphenicol respectively. Results: 55.26% of analyzed samples were contaminated with S. aureus. 100% of Methicillin Sensitive S. aureus were resistant to nalidixic acid, 70% to bacitracin, 65% to spiramycin, and 45% to penicillin and fosfomycin. There was no resistance to vancomycin, chloramphenicol, gentamicin and pristamycin among isolates. A total of 76% of the isolated strains were found to be resistant to at least 4 antibiotics. One Methicillin Resistant S. aureus strain (4.76%) was detected and showed multiple drug resistance. This resistance was crossed with all beta lactams and its resistance profile to macrolides was constitutive (MLSB const) while aminoglycosides phenotype was ANT (4’) (4’’). Conclusion: A high prevalence of S. aureus with multiple drug resistance was established. Improved food safety measures are thus necessary to prevent transmission and spread of antimicrobial resistance by these pathogens.
Authors and Affiliations
W. Chaalal, H. Aggad, K. Zidane, N. Saidi, M. Kihal
Keywords
Related Articles
- EP ID EP350844
- DOI 10.9734/BMRJ/2016/24064
- Views 106
- Downloads 0
A New Nomenclature for Cry1Ab Proteins Reflecting 3-D Structure Differences
Cry1Ab proteins produced by the insecticidal bacterium Bacillus thuringiensis are mostly studied and applied, facing the challenge of insect resistance. The 3-D structure of the toxic core for all available 34 Cry1Ab pro...
Ventilator Associated Pneumonia in a Tertiary Care Hospital: Incidence, Risk Factors and Etiological Agents
Aims: With an escalating mortality rate reaching 50%, ventilator associated pneumonia (VAP) continues to pose an enormous threat to ICU patients worldwide. Study Design: Prospective cohort study. Place and Duration: T...
Smokeless Tobacco – Ammunition against Dental Caries? A Microbial Study
Aims: The tobacco plant, Nicotiana tabacum, has been one of the major risk factors for oral cancer. However, the literature has endowed it as “holy herb” since the pre-Columbian era as being used for treating pain, poiso...
Microorganisms Associated with the Production of a Nigerian Fermented Beverage, ‘Agadagidi’
Aim: The microbial types, occurrence, loads and interactions were studied during the production of a Nigerian fermented beverage, ‘Agadagidi’, from overripe plantains. Place and Duration of Study: Federal University of...
Production and Microbiological Assesment of Date Palm (Phoenix dactylifera L.) Fruit Wine
Aim of the Study: This study was undertaken to assess the possibility of using date palm fruit (Phoneix dactylifera L.) for wine production. Study Design: Good quality, fresh and dried date fruit were gotten from Kankia...