Antimicrobial susceptibility testing of helicobacter pylori isolated from patients suffering from gastroduodenal ulcers in a tertiary care hospital
Journal Title: Indian Journal of Microbiology Research - Year 2018, Vol 5, Issue 2
Abstract
Introduction: The association of Helicobacter pylori with peptic ulcers has gained its importance and has led to ongoing research for the most appropriate treatment. Combinations of multiple drugs presently are having best cure rates. Materials and Methods: Based on clinical signs and symptoms, 96 patients were selected for study purpose, out of which 34 patients were positive for Helicobacter pylori. Identification was done based on Direct Gram stain, Gram stain of the colony, positive oxidase, catalase and urease test. Antimicrobial susceptibility testing was carried out with agar dilution method using 5-drugs namely clarithromycin, levofloxacin, metronidazole, amoxicillin and tetracycline. Results: Out of the 34 isolates, 6 isolates were resistant to clarithromycin (17.6%), 5 isolates were resistant to levofloxacin (14.7%), 20 isolates showed resistance to metronidazole (58%), only one isolate showed resistance to amoxicillin (2.9%) and 2-isolates showed resistance to tetracycline (5.8%) . In this study higher resistance was seen to metronidazole as the drug is widely used in India for the treatment of Amoebic dysentery. Compared to males, female patients showed higher resistance to metronidazole (92%) due to its use in the treatment of trichomonas vaginalis infection. Conclusion:Most patients are prescribed initial Helicobacter pylori eradication treatment without culture and antimicrobial susceptibility testing. Emergence of resistance is thought to be due to prior antibiotic use hence irrational use of antimicrobial should be stopped to prevent drug resistant.
Authors and Affiliations
Vinay Hajare, Mohammed Abdul Hadi Waseem, Aaftab G P.
Keywords
Related Articles
- EP ID EP472796
- DOI 10.18231/2394-5478.2018.0037
- Views 69
- Downloads 0
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