Antimutagenic Effects of Ascorbic Acid against the Genotoxicity of Dimethyl Sulphate in Drosophila
Journal Title: Universal Journal of Environmental Research and Technology - Year 2012, Vol 2, Issue 1
Abstract
The present work involves the study of the genotoxicity of an alkylating agent, dimethyl sulphte, and the possible protective efficacy of vitamin C against the toxicity of this chemical in the somatic cells (wing primordial) of Drosophila melanogaster following chronic larval exposures. For the wing mosaic assay two high bioactive strains of Drosophila melanogaster, viz., ORR; mwh/mwh and ORR; flr3/TM3, Ser were used. Third instar larvae of flr3/TM3, Ser and mwh homozygous male cross were exposed to the LD50 and lower doses of dimethyl sulphate (DMS) to find out its genotoxicity. Concurrent negative control was run simultaneously in each experiment, where the larvae were exposed to the solvent (distilled water). All the doses of DMS were found to be clearly genotoxic in Drosophila wing mosaic assay in comparison to the control. The frequencies of induction of small and large single spots were positive. With respect to the frequency of twin spots, the statistical outcome was positive at the highest dose and inconclusive at the three lower doses. When the larvae were concurrently treated with the mixture of 300mM of ascorbic acid and the different doses of dimethyl sulphate, except for the outcomes being weak for the small singles at 100mM and 50mM of DMS, the same for these spots at the two lower doses as well as for other types of spots at all the doses were negative compared to the DMS alone. In the present experiments the results clearly indicate antimutagenic and/or anticarcinogenic action of ascorbic acid against the mutagenicity of dimethyl sulphate irrespective of the mechanisms involved.
Authors and Affiliations
Mohanty Bidut Prava, Mahananda Malaya Ranjan, Tripathy Niraj Kanti
Keywords
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