Antimutagenicity and phytoconstituents of Egyptian Plantago albicans L.
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2014, Vol 4, Issue 0
Abstract
Objective: To evaluate the safety and the possible antimutagenic effect of the defatted aqueous ethanol extract and polysaccharide extract of Plantago albicans L. areal parts on the genotoxicity of the anticancer drug cyclophosphamide and to investigate the chemical constituents of these extracts. Methods: Two doses of each extract (10 and 15 mg/kg body weight) were administered orally to albino mice 7 d prior to treatment of cyclophosphamide (20 mg/kg body weight, i.p). Chromosomal aberration analysis and micronucleus test were performed. Phytochemical investigation of defatted aqueous ethanol extract was carried out through chromatographic tools, chemical and physical analysis to know the active constituents of these extracts. Results: Groups of mice administered with defatted aqueous ethanol extract and polysaccharide extract prior to cyclophosphamide treatment showed statistically significant reduction in the percentage of chromosomal aberrations and micronuclei in bone marrow cells in a dosedependent manner. Defatted aqueous ethanol extract gave more effective protection in comparison with polysaccharide extract. Phytochemical investigation of defatted aqueous ethanol extract allowed isolation of gallic acid, methylgallate, tri, tetra, penta-galloyl-β- glucopyranoside, kaempferol-3-O-β-sophro pyranoside, quercetin-3-O-β-glucopyranoside-7- O-α-rhamnpyranoside, kaempferol and quercetin, for the first time from this species. Conclusions: The results of the present work demonstrated that Plantago albicans phytoconstituents play a protective role against genotoxicity of the drug cyclophosphamide.
Memory-enhancing activity of Anacyclus pyrethrum in albino Wistar rats
Objective: To explore the potential effect of ethanolic extract of Anacyclus pyrethrum (A. pyrethrum) in memory dysfunction. Methods: Memory impairment was produced by administration of scopolamine (1mg/kg i. p) in rats....
Evaluation of Ashwagandha in alcohol withdrawal syndrome
Objective: To evaluate the effect of Ashwagandha (ASW) in attenuation of alcohol withdrawal in ethanol withdrawal mice model. Methods: Alcohol dependence was induced in mice by the oral, once-daily administration of 10%...
Role of behavioural communication change in integrated vector management against vector borne diseases especially dengue in Punjab, Pakistan
No
Middle East respiratory syndrome (MERS 2012): roles of clinical pathology laboratory for screening and diagnosis
no
Isolation of Shigella species and their resistance patterns to a panel of fifteen antibiotics in mid and far western region of Nepal
Objective: To determine the antimicrobial resistance patterns of Shigella species to the most commonly used antibiotics in mid and far western part of Nepal. Methods: Stool samples were collected from 458 patients who ca...