Antioxidant Activity of Flavonoids Isolated from Rosmarinus officinalis L.
Journal Title: Journal of Plant Science and Research - Year 2016, Vol 3, Issue 1
Abstract
Rosmarinus officinalis L. (Lamiaceae), Rosemary is recognized several years ago in the traditional medicine by its therapeutic virtues and its ability to treat some diseases, in this context the present study aim to determine the content of phenolic and flavonoid compounds contained in Rosmary leaves and to evaluate their antioxidant activity. The total phenolic and flavonoid contents were estimated by the methods of Folin-Ciocalteu and AlCl3 methods respectively. Diagnosis and isolation of flavonoids have been carried out by various chromatographic techniques (Thin layer chromatography and Column chromatography) and their structural identification was carried out by spectral analysis by the spectrophotometer (UV-Vis). The antioxidant capacity was evaluated by in vitro trapping of free radical DPPH. The total polyphenols and flavonoids contents in rosemary were 10.42 mg GAE/g of dry material for polyphenols and 9,075±0,002 mg QE/g of dry material for flavonoids. The chromatographic and spectral identification of extracts of thyme revealed the presence of three flavonoids characterized by their antioxidant activity in the order of 90%, 88%, 87% respectively for quercetin 3OR, quercetin, and luteolin compared to quercetin standard characterized by its percentage of scavenging DPPH equivalent to 93.05%. In conclusion, we report that the flavonoids of R. officinalis exhibit good antioxidant activity against free radicals. The molecules may be used as antioxidants and for therapeutic applications.
Authors and Affiliations
Zeghad Nadia
Keywords
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