Antioxidant activity, total phenolic and total flavonoid contents of stem back and root methanolic extracts of Calotropis procera
Journal Title: The Journal of Phytopharmacology - Year 2019, Vol 8, Issue 4
Abstract
Calotropis procera it is known for traditional Kenyan medicinal system and it is used for it has previously been employed for treatment various diseases. This study aimed at examining methanolic extract of C. procera to establish antioxidant potential in vitro, total phenolic and flavonoid contents in order to identify potential sources of new antioxidants in food and pharmaceutical formulations. A comprehensive assessment on the antioxidant activity of stem back and root of C. procera by in vitro chemical analyses. Total phenolic, total flavonoid contents and total yield of the samples were also estimated. Preliminary phytochemical tests were also carried out to establish the components of plant extracts. Results of the study revealed presence of saponins terpenoids, alkaloids, anthraquinones and steroids. The stem back extract had significantly higher amounts of total phenol and flavonoid contents (79.80 ± 3.79 mg GAE/g extract and 71.63±4. mg RTE/g extract, respectively) compared to root samples. We established correlation between total phenolic contents and EC50 values for DPPH free radical scavenging property and reducing power of extract, ABTS radical cation and phosphomolybdate. Taken together, this work demonstrated considerable protective effectiveness in C. procera stem back and root methanolic extracts that function as an antioxidizing agent due to their free radical scavenging activity.
Authors and Affiliations
Wilton Mbinda, Colleta Musangi
Keywords
Related Articles
- EP ID EP49941
- DOI http://www.phytopharmajournal.com/Volume8issue4.html#
- Views 225
- Downloads 0
Standardization of Haridradi churna- physicochemical assay and HPTLC profile
Haridradi churna, a polyherbal preparation containing Haridra (Curcuma longa Linn.), Devadaru (Cedrus deodar Roxb), Lodhra (Symplocos racemosa Roxb), Priyangu (Callicarpa macrophylla Vahl) and Yastimadhu (Glycyrrhiza g...
Anticonvulsant actions of ethanol stem bark extract of Trichilia roka (Meliaceae) in mice and chicks
The current antiseizure drugs present with side effects, some of which can lead to discontinuation of epilepsy pharmacotherapy, and a sizeable number of other drugs being less efficacious. Medicinal plants are used for...
Natural product medicines: A literature update
Natural products represent major approach for the discovery and development of new drugs. An updated review on the natural products is lacking in the literature. So, in the present review, an attempt has made to pool t...
Evaluation of anticancer property of mango peel and flesh after formalin treatment
Cancer is life threatening disease an d the second leading cause of death. Various phenolic compounds obtained from plant sources such as fruit, bark, leaf and roots acts against cancer but when such fruits are treated...
Screening for community-acquired strains of methicillinresistant Staphylococcus aureus susceptible to extracts of Centaurea nigrescens
The rates of infection by community-acquired multi-drug resistant Staphylococcus aureus have risen dramatically over fifteen years in the United States. Community-acquired multi-drug resistant Staphylococcus aureus is...