Antioxidant and α-glucosidase activities and phytochemical constituents of Chrysanthoglossum trifurcatum (Desf.)
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 4
Abstract
Objective: To investigate the antioxidant and α-glucosidase properties and phytochemical constituents of roots, stems, leaves and flowers extracts and aerial parts oil of Chrysanthoglossum trifurcatum (Desf.) (C. trifurcatum). Methods: For extraction from roots, stems, leaves and flowers of C. trifurcatum, methanol, ethyl acetate and petroleum ether solvents were used. Phenols, flavonoids, flavonols and tannins contents were evaluated. More, C. trifurcatum aerial parts oil composition was determined using chromatography/mass spectrometry. The antioxidant effect was estimated by DPPH, ABTS and reducing power test systems. The α-glucosidase inhibition was determined by colorimetric assay using the enzyme from Aspergillus niger and the p-nitrophenyl glucopyranoside (pNPG) as substrate. Results: The highest amounts of polyphenols, flavonoids, flavonols and tannins were shown by the methanolic extract of leaves. The main components of the aerial parts oil were limonene (29.21%), γ-terpinene (12.96%), 4-terpenyl acetate (12.18%) and α-pinene (5.76%). The activity evaluated by DPPH, ABTS and reducing power tests was important for stems (IC50=0.68 mg/mL) and flowers (IC50=0.67 mg/mL) methanolic extracts and essential oil (IC50=0.72 mg/mL). Findings of α-glucosidase activity revealed that petroleum ether extracts of leaves and roots together with aerial parts oil showed a highest activity with IC50 of 0.044, 0.045 and 0.049 mg/mL, respectively. Conclusions: Observed antioxidant and α-glucosidase activities of oil and extracts are attributed to the presence of the active phytochemicals in C. trifurcatum organs. Thus, the C. trifurcatum can be used as a source of antioxidant compounds and dietary supplement to treat patients with type 2 diabetes.
Authors and Affiliations
Ahlem Ben Sassi
Keywords
Related Articles
- EP ID EP283771
- DOI 10.4103/1995-7645.231469
- Views 86
- Downloads 0
Bacillomycin D-like compounds isolated from Bacillus amyloliquefaciens HAB-2 are effective against Burkholderia pseudomallei
Background: Burkholderia pseudomallei (Bp) is a gram-negative environmental bacterium that causes melioidosis. It has high mortality and relapse rates regardless of powerful antibiotic therapy. Bacterial pathogens displa...
Effect of dietary supplementation with olive and sunflower oils on lipid profile and liver histology in rats fed high cholesterol diet
Objective: To compare the effects of high-monounsaturated (MUFA) and polyunsaturated fatty acids (PUFA) against the metabolic disorders elicited by a highcholesterol diet (HC) in rats. Methods: Using in vivo dietary mani...
Immune enhancement effect of an herb complex extract through the activation of natural killer cells and the regulation of cytokine levels in a cyclophosphamide-induced immunosuppression rat model
Objective: To investigate the effects of a herb complex extract (HCE) prepared from Cornus officinalis Sieb. Et Zucc., Eriobotrya japonica Lindley, and olive leaves on immune response of mouse spleen NK cells in vitro an...
Phytocompounds of Anonna muricata leaves extract and cytotoxic effects on breast cancer cells
Objective: To identify the phytochemical compounds from Annona muricata (A. muricata) and to determine their in vitro anti-proliferative activities against breast cancer cells, MCF7 and MDA-MB-231. Methods: A. muricata l...
Seronegative disseminated Bartonella spp. infection in an immunocompromised patient
An 11 year old, hispanic girl with a history of B-cell acute lymphoblastic leukemia was admitted to the hospital for symptoms compatible with Bartonella henselae infection. The first molecularly diagnosed case of dissemi...