ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF RUBUS FRUTICOSUS AND ZIZYPHUS VULGARIS METHANOL EXTRACTS
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 2
Abstract
Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo.Methods: Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability.Results: The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.Conclusion: The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents.
Authors and Affiliations
Asma Meziti, Hamama Bouriche, Meziti Hichem, Seoussen Kada, Abderrahmane Senator, Ibrahim Demirtas
Keywords
Related Articles
- EP ID EP575823
- DOI 10.22159/ijpps.2017v9i2.14374
- Views 92
- Downloads 0
ANTIMICROBIAL ACTIVITY OF SYZYGIUM CALOPHYLLIFOLIUM WALP. LEAF EXTRACT
Objective: To evaluate the antimicrobial activity of methanol extract of Syzygium calophyllifolium Walp. leaf against the gram-negative bacteria, namely, Escherichia coli, Klebsiella pneumonia, Salmonella typhi, Proteus...
ULTRA LOW POWER HYBRID MICRO ENERGY HARVESTER USING RF, THERMAL AND VIBRATION FOR BIOMEDICAL DEVICES
The objective of this research is to design ultra-low power Hybrid Micro Energy Harvester (HMEH) circuit using hybrid inputs of radio frequency (RF), thermal and vibration for biomedical devices. In the HMEH architecture...
BIOLOGICAL SCREENING OF ARAUCARIA COLUMNARIS
Objective: The present research is biological screening of aerial part of Araucaria columnaris (Araucariaceae). There were investigation for their antibacterial, antifungal, phytotoxic and cytotoxic activities of Dichlor...
EPIGENETICS: EFFECT OF ENVIRONMENTAL FACTORS ON HUMAN GENOME
The physical and social behavior of an organism is influenced by its epigenetic modification, which involved histone modification, DNA methylation, non-coding RNA expression and chromatin remodeling. In contrast to genet...
CYTOMORPHOLOGY OF SOME MEDICINAL GRASSES FROM HANGRANG VALLEY OF DISTRICT KINNAUR, HIMACHAL PRADESH
Objective: The present study is focused on the chromosome number, their meiotic behavior, pollen fertility and medicinal importance of 6 species of grasses from the remote high altitude region of Kinnaur, Himachal Prades...