ANTIOXIDANT, ANTI-LIPID PEROXIDATIVE AND ANTIMICROBIAL PROPERTIES OF THE EPIPHYTIC FERN, PYRROSIA HETEROPHYLLA (L.) M. G. PRICE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 4
Abstract
Objective: The present study was aimed at establishing the antioxidant, free radical scavenging, anti-lipid peroxidative and antimicrobial properties of the plant Pyrrosia heterophylla (L.) M. G. PRICE.Methods: Standard protocols were used to estimate the antioxidant potential of the hexane, ethyl acetate and methanolic extracts of the plant. Radical scavenging ability of the extracts was assayed for 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS), 1,1-diphenyl-2-picryl-hydrazil (DPPH) and hydroxyl radicals. Total antioxidant activity assay was done following the phospho- molybdenum method. The reductive potential was measured by ferric reducing antioxidant power (FRAP) assay. Lipid peroxidation assay was done in vitro. Total phenolic content was measured by the Folin-Ciocalteu method. Antimicrobial activity was identified by well diffusion method, and minimum inhibitory concentration (MIC) was determined by serial dilution method.Results: Results revealed that the ethyl acetate extract (PHE) exhibited the highest antioxidant capacity followed by the methanolic extract (PHM) whereas the hexane extract (PHH) had the lowest activity. The percentage radical scavenging by PHE was found to be 86.63±0.85, 89.48±2.08 and 70.89±1.46 for DPPH, ABTS and hydroxyl radicals respectively, at a concentration of 800μg/ml. The total antioxidant activity of PHE, PHM and PHH was found to be 538.33±3.51, 283.33±7.57and 13.76±3.95 ascorbic acid equivalents/g of extract respectively. Phenolic content of PHE was the highest (207.22±1.95 gallic acid equivalents (GAE)/g of extract), followed by PHM and PHH (197.92±2.00 and 37.50±2.18 GAE/g respectively). Total reducing power was also found to be the highest in PHE followed by PHM and PHH as per the FRAP assay. All the extracts were found to possess inhibitory activity against the tested microorganisms. MIC50 value of all the extracts was below 40 µg/ml.Conclusion: The results of this study confirmed the antioxidant, antimicrobial and anti-lipid peroxidation potentials of the plant P. heterophylla (L.) M. G. PRICE.
Authors and Affiliations
Meera George, Leenamma Joseph, V. S. Josekumar
Keywords
Related Articles
- EP ID EP575685
- DOI 10.22159/ijpps.2017v9i4.16827
- Views 46
- Downloads 0
NANOEMULSION GEL OF NUTRACEUTICAL CO-ENZYME Q10 AS AN ALTERNATIVE TO CONVENTIONAL TOPICAL DELIVERY SYSTEM TO ENHANCE SKIN PERMEABILITY AND ANTI-WRINKLE EFFICIENCY
Objective: The object of our investigation was to develop and characterize nanoemulsion gel (NEG) as transdermal delivery systems for the poorly water soluble drug, Co-enzyme Q10 (CoQ10), to improve its solubility and sk...
DEVELOPMENT AND VALIDATION OF STABILITY-INDICATING RP-HPLC ASSAY METHOD FOR AZACITIDINE AND ITS BULK DRUG
Objective: A novel gradient reverse phase High Performance Liquid Chromatographic (HPLC) method was developed for the quantification of Azacitidine impurities and degradation products in the Azacitidine tablets.Methods:...
DESIGN OF COX-2 INHIBITORS–AN IN-SILICO APPROACH
Objective: The aim of the present work was to design the novel series of chalcone derivatives of indane-1,3-dione for its inhibition towards COX-2.Methods: COX-2 inhibitors were designed on the binding ability of the com...
PREPARATION AND CHARACTERIZATION OF ORAL NANOSUSPENSION LOADED WITH CURCUMIN
Objective: The principle objective of the present research work was to improve the bioavailability of curcumin (CUR) by decreasing its particle size. Nanosuspension (NS) of CUR was prepared using poloxamer-188 (P188) as...
SYNTHESIS AND EVALUATION OF OLMESARTAN MEDOXOMIL COMPLEX WITH SBE7 β-CD FOR ENHANCED DISSOLUTION AND BIOAVAILABILITY
Objective: Olmesartan is a BCS class II anti-hypertensive drug with limitations of low aqueous solubility and bioavailability. ÂÂPresent work was designed to use complexation as an approach to explore the ability of mo...