ANTIOXIDANT, ANTIMICROBIAL, ANTIPROLIFERATIVE ACTIVITIES AND IN SILICO DRUG LIKENESS PREDICTION OF PURIFIED MHH COMPOUND, ISOLATED FROM MILLINGTONIA HORTENSIS LINN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: To investigate the antioxidant, antimicrobial, antiproliferative activities and in silico drug likeness prediction of the MHH compound.Methods: The antioxidant, antimicrobial and antiproliferative activities of the MHH compound have been evaluated by 1,1-Diphenyl-2-picryl hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays, agar well diffusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay respectively. The cytotoxicity activity and in silico drug likeness prediction of the MHH compound have been studied.Results: The DPPH radical scavenging activity and the total antioxidant power of the MHH compound were found to be 92.48%±2.06 and 10.698±0.23 mM Fe (II)/g respectively. The antimicrobial activity of MHH compound against Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Staphylococcus aureus and Streptomyces griseus, Candida albicans, Aspargillus flavus and the zones of inhibition were found to be 16.83 mm, 19.83 mm, 15.23 mm, 16.79 mm, 13.57 mm, 19.65 mm, 18.66 mm and 14.79 mm respectively. The IC50 values of the MHH compound for HeLa and HCT-15 cell lines were found to be 371.92 μg/ml and 112.06 μg/ml respectively. The LC50 value of MHH compound was 218.765µg/ml and 152.78µg/ml which is considered as moderately cytotoxic and toxic respectively when incubated at 24 h and 48 h. The drug likeness and bioactivity scores of the MHH compound were found to be within the range and followed Lipinski’s rule.Conclusion: This study concludes that the MHH compound has significant biological activities and can be used as the therapeutic agent.Â
Authors and Affiliations
Mahesh Kumar Mvs, Rajagopal S V
Keywords
Related Articles
- EP ID EP578440
- DOI -
- Views 69
- Downloads 0
ANTIOXIDANT THERAPEUTIC ACTIONS OF MEDICINAL PHYTOCHEMICALS, SILYMARIN AND SILIBININ, ON STREPTOZOTOCIN DIABETIC RATS: FIRST NOVEL COMPARATIVE ASSESSMENT OF STRUCTURAL RECOVERIES OF HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES ON ISLETS OF LANGERHANS, Î’-CELLS, MITOCHONDRIA AND NUCLEUS
Objective: We studied correlation between antioxidant properties of Silymarin and Silibinin and the restoration and recovery of normal structure of islets of Langerhans β-cells and mitochondria in Streptozotocin-diabeti...
SCREENING AND CHARACTERISTIC STUDY OF ANTIMICROBIAL ACTINOMYCETES FROM NEAR-BY SOIL OF MEDICINAL PLANTS
Objective: Isolation and characterization study of actinomycetes from soil near medicinal plants in saurashtra region of Gujarat which can produce antimicrobial compounds.Methods: Present study 11 different medicinal pla...
SPICY ANTI-CANCER SPICES: A REVIEW
The uses of ethnomedicinal dietary antimutagens and anticarcinogens for human healthcare still remain the most promising approach to the protection of human health and medication system in developing and under developing...
ANTI-TUMOR POTENTIAL OF ERVATAMIA DIVARICATA. L. BURKILL AGAINST EHRLICH ASCITES CARCINOMA
Objective: The present study aims to evaluate the anti-tumor potential of EEED (ethanolic extract of Ervatamia divaricata. L. Burkill) on EAC (Ehrlich ascites carcinoma).Methods: The ethanolic extract of Ervatamia divari...
IN VITRO ANTIOXIDANT AND ANTICANCER POTENTIAL OF FLOWERS OF TODDALIA ASIATICA (RUTACEAE)
Objectives: The aim of this research was to evaluate the phytochemical, antioxidant and anti cancer property of the methanol extract of flowers of Toddalia asiatica.Methods: The phytochemical screening of the methanol ex...