ANTIOXIDANT, ANTIMICROBIAL, ANTIPROLIFERATIVE ACTIVITIES AND IN SILICO DRUG LIKENESS PREDICTION OF PURIFIED MHH COMPOUND, ISOLATED FROM MILLINGTONIA HORTENSIS LINN
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 9
Abstract
Objective: To investigate the antioxidant, antimicrobial, antiproliferative activities and in silico drug likeness prediction of the MHH compound.Methods: The antioxidant, antimicrobial and antiproliferative activities of the MHH compound have been evaluated by 1,1-Diphenyl-2-picryl hydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays, agar well diffusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay respectively. The cytotoxicity activity and in silico drug likeness prediction of the MHH compound have been studied.Results: The DPPH radical scavenging activity and the total antioxidant power of the MHH compound were found to be 92.48%±2.06 and 10.698±0.23 mM Fe (II)/g respectively. The antimicrobial activity of MHH compound against Escherichia coli, Klebsiella pneumonia, Proteus vulgaris, Salmonella typhi, Staphylococcus aureus and Streptomyces griseus, Candida albicans, Aspargillus flavus and the zones of inhibition were found to be 16.83 mm, 19.83 mm, 15.23 mm, 16.79 mm, 13.57 mm, 19.65 mm, 18.66 mm and 14.79 mm respectively. The IC50 values of the MHH compound for HeLa and HCT-15 cell lines were found to be 371.92 μg/ml and 112.06 μg/ml respectively. The LC50 value of MHH compound was 218.765µg/ml and 152.78µg/ml which is considered as moderately cytotoxic and toxic respectively when incubated at 24 h and 48 h. The drug likeness and bioactivity scores of the MHH compound were found to be within the range and followed Lipinski’s rule.Conclusion: This study concludes that the MHH compound has significant biological activities and can be used as the therapeutic agent.Â
Authors and Affiliations
Mahesh Kumar Mvs, Rajagopal S V
Keywords
Related Articles
- EP ID EP578440
- DOI -
- Views 61
- Downloads 0
A MUCOADHESIVE GASTRORETENTIVE DOSAGE FORM FOR VALACYCLOVIR
Objective: The objective of the present work was to formulate a gastroretentive dosage form of Valacyclovir, an anti viral drug.Methods: Valacyclovir gastroretentive mucoadhesive tablets were prepared using polymers such...
TRIPLE OBJECTIVE OPTIMIZATION OF CYTOTOXIC POTENCY OF HUMAN CARCINOMA CELL LINES OF A MARINE MACROALGAE USING NON-SORTING GENETIC ALGORITHM–A THEORETICAL STUDY
Objective: The main purpose of our work was to arrive at an acceptable model for optimizing the cytotoxic potency of Ulva fasciata Delile extract on human carcinoma cell lines of which can provide believable indications...
ANTI-QUORUM SENSING ACTIVITY OF SOME COMMONLY USED TRADITIONAL INDIAN SPICES
Objective: To investigate the total phenolic and flavonoids contents and study the anti-quorum sensing activity of Indian spices.Methods: The methanolic extracts of eight Indian spices were evaluated for phytochemicals a...
IN VITRO PLASMID CURING ACTIVITY OF AQUEOUS EXTRACT OF TERMINALIA CHEBULA FRUIT AGAINST PLASMIDS OF BACILLUS SUBTILIS AND SHIGELLA SONNEI
Objective: The aim of this investigation was to evaluate in vitro plasmid curing activity of the aqueous extract from Terminalia chebula fruit against multi-drug resistance bacteria.Methods: The aqueous extract of Termin...
ISOLATION, SCREENING AND IDENTIFICATION OF CEFDINIR DEGRADING YEASTS FOR THE TREATMENT OF PHARMACEUTICAL WASTEWATER
Objective: The aim of the present study was to isolate, screen and identify the cefdinir degrading yeasts for the treatment of pharmaceutical wastewater.Methods: The steps include isolation of yeasts from pharmaceutical...