ANTIOXIDANT EFFECT OF AQUEOUS EXTRACT OF MUCUNA PRURIENS (LINN) BY DPPH ASSAY- AN IN-VITRO STUDY
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2018, Vol 7, Issue 8
Abstract
BACKGROUND In human, oxidative stress has been proposed in the pathogenesis of cardiovascular diseases, neurodegenerative disorders like Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis, male and female infertility etc. Objective- The present study was carried out to determine the antioxidant potential of Mucuna pruriens using DPPH assay. MATERIALS AND METHODS The seeds of M. pruriens were powdered and aqueous extract was prepared. DPPH (1, 1-diphenyl-2-picrylhydrazyl) is a stable free radical. The unpaired electron delocalises over the molecule entirely and the molecules will not undergo dimerisation similar to majority of free radicals. This process resulted in formation of deep purple colour, characterised by an absorption band in ethanol solution. The absorbance of all samples was read at 517 nm using UV visible spectrophotometer. Antioxidant activity was calculated. Each step was carried out in triplicate and the average values were entered. The experimental results were expressed as mean ± SD of three parallel measurements. IC50 (minimum inhibitory concentration) values were calculated graphically. RESULTS The DPPH scavenging activity of aqueous extract of Mucuna pruriens was recorded in terms of percentage inhibition. The results obtained were comparable to the standard BHT (Butylated Hydroxytoluene) in a dose dependent manner. Higher percentage scavenging indicates greater antioxidant potential; 1000 micrograms of AEMP shows greatest scavenging activity. CONCLUSION When there is an excessive generation of free radicals, it can result into oxidative stress which gets accumulated during the life span which has been contributed in long-term ailments, therefore it is vital to restrict oxidative injury to tissues and suppress progression of the disease by supplying antioxidants as nutraceuticals. The current study concludes that the aqueous extract of seeds of Mucuna pruriens demonstrated considerable antioxidant activity.
Authors and Affiliations
Akila Lakshmikandhan, Ramya Ravichandar
Keywords
Related Articles
- EP ID EP410131
- DOI 10.14260/jemds/2018/237
- Views 79
- Downloads 0
A COMPARATIVE STUDY OF THE POST-OPERATIVE MATERNAL COMPLICATIONS OF ELECTIVE VERSUS EMERGENCY LOWER SEGMENT CAESAREAN SECTION- A SECONDARY DATA ANALYSIS
BACKGROUND Caesarean delivery is defined as the birth of a foetus through incisions in the abdominal and uterine wall. Several studies conducted across India have shown alarming increase in the rate of caesarean section...
COEXISTENCE OF PEMPHIGUS VULGARIS AND VITILIGO VULGARIS IN A PATIENT OF CHRONIC LIVER DISEASE- A POINTER TOWARDS COMMON AUTOIMMUNE AETIOLOGY
A 56-year-old gentleman with stable vitiligo vulgaris for the past 20 years presented with flaccid vesicles and raw, painful erosions on various parts of body. These lesions erupted on and off for the past 2 years and he...
STUDY ON PREGNANCY OUTCOME IN PRETERM PREMATURE RUPTURE OF MEMBRANES WITH OLIGOHYDRAMNIOS
BACKGROUND Pregnancy outcome in preterm premature rupture of membranes is affected by AFI. PPROM with oligohydramnios (AFI<5) affects maternal outcome in terms of latency period, complications like chorioamnionitis, mode...
IMPLEMENTATION OF ‘5S’ TECHNIQUES IN A TERTIARY CARE TEACHING HOSPITAL
BACKGROUND 5S is considered as a foundation for lean, because of its ability to eliminate wastes from non-value added activities or waste from human motion. It is a structured program to systematically organise workplace...
NEWER MANAGEMENT TIPS FOR PHACOEMULSIFICATION OF BRUNESCENT NUCLEI
BACKGROUND Phacoemulsification is the standard technique for elective cataract surgery worldwide. In spite of recent advances in techniques and technology, the management of black and brunescent cataracts remains a chall...