Antioxidant effect of hyaluronan on polymorphonuclear leukocyte-derived reactive oxygen species is dependent on its molecular weight and concentration and mainly involves the extracellular space
Journal Title: Advances in Hygiene and Experimental Medicine - Year 2009, Vol 63, Issue
Abstract
Introduction: Hyaluronan (HA), a component of the extracellular matrix, may regulate immune cell functions through its interactions with cellular receptors. Besides its effect on cytokine and chemokine production, its antioxidant properties have been described. However, the mechanisms of this are not fully elucidated. The aim of this study was to evaluate the relationship between HA concentration and molecular weight and its antioxidant properties towards human neutrophils. Also assessed was whether the antioxidant effect of HA is connected with a reduction in intracellular oxygen potential, which could indicate its direct effect on neutrophil respiratory burst.Materials/Methods: The relationship between HA’s antioxidant properties and its concentration and molecular weight was assessed by the luminol-enhanced chemiluminescence method (CL). To evaluate the effect of HA on intracellular oxygen potential selectively, the dihydrorhodamine 123 (DHR123) flow cytometric method was used.Results: Reduction of both HA molecular weight and its concentration decreased its antioxidant properties in the CL method. A selective effect of HA on intracellular oxygen potential measured by the DHR123 method was not shown.Conclusions: The antioxidant properties of HA are related to both its molecular weight and its concentration. The lack of an antioxidant effect of HA in the DHR123 test compared with a significant reduction in CL values at the same HA concentration suggests that HA acts mainly as a chemical ROI scavenger in the extracellular space.
Authors and Affiliations
Rafał Krasiński, Henryk Tchórzewski, Przemysław Lewkowicz
Keywords
Related Articles
- EP ID EP66326
- DOI -
- Views 159
- Downloads 0
Yeast as a model for studying neurodegeneration
At the level of genetics and physiology the yeast Saccharomyces cerevisiae is are the best characterized eukaryotic cells. The yeast cells can be used as a model to study the mechanisms involved in human disease. Yeast...
Wpływ naturalnych białek serwatki na mechanizmy regulacji ciśnienia tętniczego krwi
Serwatka jest bogatym, naturalnym źródłem białek i aminokwasów. W licznych badaniach wykazano, że biologicznie aktywne peptydy wyizolowane z serwatki uzyskanej z mleka krowiego mogą wpływać na regulację ciśnienia tętnicz...
Rola metalotioneiny w procesie nowotworzenia oraz w leczeniu chorób nowotworowych
Metalotioneina to niskocząsteczkowe białko bogate w grupy sulfhydrylowe. Reszty cysteinowe metalotioneiny odgrywają główną rolę w procesach fizjologicznych, takich jak: homeostaza pierwiastków niezbędnych, detoksykacja m...
Renal catabolism of albumin – current views and controversies
Albumin is the main protein of blood plasma, lymph, cerebrospinal fluid and interstitial fluid. The protein assists in many important body functions, including maintenance of proper colloidal osmotic pressure, transport...
Evolution of sanitary-epidemiological services in Poland in the years 1944-2014
This paper presents the history of sanitary-epidemiological services in the context of the health, economic and socio-political situation in Poland in the years 1944-2014, with a critical analysis of legal restraints, ef...