Antioxidant Effects of Graptophyllum pictum Leaf Extract on Malondialdehyde (MDA) Levels of Mice Induced By a Toxic Dose of Paracetamol
Journal Title: Journal of Krishna Institute of Medical Sciences University - Year 2018, Vol 7, Issue 3
Abstract
Background: Antioxidants are important substances which possess the ability to protect the body from damage caused by free radicals-induced oxidative stress. A shift in the balance between oxidants and antioxidants promotes oxidative stress. Graptophyllum pictum (GP) contains substances that are efficacious as antioxidants. Aim and Objectives:To prove the antioxidant effects of GP leaf extract on Malondialdehyde (MDA) levels of mice induced by a toxic dose of paracetamol. Material and Methods: Thirty mice were randomly divided into five groups (n=6); Negative Control (NC) was without treatment, Positive Control (PC) with aquadest + paracetamol, T1: GP leaf extract 150 mg/kg BW + paracetamol, T2: GP leaf extract 300 mg/kg BW+ paracetamol, T3: GP leaf extract 600 mg/kg BW+ paracetamol. Extracts were given on the 1st to 10th day and paracetamol induction was performed on the 8th, 9th, and 10th days. On the 11th day, blood serum samples were collected and the level of MDA was then measured Results: T1, T2, T3 had the lower number of MDA levels when compared with the PC (3.625±0.374, 3.147±0.222, 2.574±0.319). One way Analysis of Variance (ANOVA) and post hoc test with Tukey showed that there was a significant difference between PC and T1, T2, T3 (p<0.05). Conclusion: GP extract has an antioxidant effect on the prevention of elevated MDA levels. A dose of 600 mg/kg BW was found to be the most effective in preventing elevation of MDA levels after a toxic dose of paracetamol was induced.
Authors and Affiliations
Tuti Kusumaningsih, Anita Firdausi, Indeswati Diyatri, Rini D. Ridwan, Ira Arundina, Yuliati .
Keywords
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