Antioxidant effects of immunomodulators in patients with recurrent pyelonephritis
Journal Title: УКРАЇНСЬКИЙ ЖУРНАЛ НЕФРОЛОГІЇ ТА ДІАЛІЗУ - Year 2018, Vol 2, Issue 58
Abstract
The purpose of our work was to investigate the effect of immunomodulatory medicines on the intensity of oxidative stress (OS), the cytokines level and the activity of renospecific enzymes in patients with recurrent pyelonephritis (rPN). Methods. A prospective, randomized, open-label study involved of 100 women aged 33.4 ± 8.8 year old. According to the sensitivity of the detected pathogens all patients received antibacterial therapy for two weeks. Along with the main course of antibiotic therapy, 25 patients were assigned Sodium nucleinate at a dose of 0.25 g 4 times per day during 14 days, 18 patients were prescribed Galavit intramuscularly 2 ml per dayfor 10 days, and 27patients were prescribed Proteflazid according to the manufacturer’s instructions. The comparison group consisted of 30 women with rPN who received antibiotic therapy exclusively.Women were screened before and after the treatment. The content of malondialdehyde (MDA), ceruloplasmin (CP), transferrin (TF) and sulfhydryl groups (SH-groups) were determined in the blood by colorimetric method. Oxidative stress index (OSI) was calculated. The concentration of interleukins (IL) -1f, -4, -8, -10, -17, tumor necrosis factor a (TNF-a), transforming growth factor f (TGF-f), monocytic chemoactive protein-1 (MCP-1) and interferon y (IFN-y) were analyzed in the blood of the women using an ELISA. To evaluate the functional state of the renal parenchyma the activity of tubular lysosome enzymes a total f-N-acetylhexosaminidase and f-galactosidase were determined in urine. Results. The use of Sodium nucleinate decreased of the OS activity by reducing MDA level (p <0.001) and increasing the concentration of CP (p < 0.03). The serum levels ofIL-4 (p = 0.007), lL-17 (p = 0.04), TGF-f (p = 0.02) and MCP-1 (p = 0.03) were decrease. The use of Galavit contributed to a statistically significant decrease in the concentration of TNF-a (p <0.001), IL-8(p <0.001), IFN-y (p = 0.001) and TGF-f (p <0.001). The administration of Proteflazid resulted in a decrease in the concentrations of IL-8 and IFN-y, with a decrease in OSI (p =0.04) compared to pre-treatment. All applied immunomodulators partially reduced the activity of renospecific enzymes markers of kidney damage. Conclusions. The use of immunomodulators in the complex therapy of patients with rPN contributes to the partial normalization of functional activity of immune system by the decreasing ofthe production ofcytokines as its mediators and the reducing ofthe OS intensity.
Authors and Affiliations
M. Kolesnyk, L. V. Korol, N. M. Stepanova, V. Driianska, L. Ya. Migal
ДОПЛЕРОГРАФІЧНА ХАРАКТЕРИСТИКА ПОРУШЕНЬ НИРКОВОГО КРОВООБІГУ У ХВОРИХ НА ХРОНІЧНУ ХВОРОБУ НИРОК І-ІІ СТАДІЇ З АРТЕРІАЛЬНОЮ ГІПЕРТЕНЗІЄЮ
Метою роботи було дослідити порушення ниркового кровообігу у хворих на хронічну хворобу нирок І-ІІ стадії (пієлонефрит, гломерулонефрит, діабетична нефропатія). Матеріали і методи: Було проведено 131 комплексне ультразв...
ПРОГНОЗИРОВАНИЕ НЕБЛАГОПРИЯТНОГО ТЕЧЕНИЯ НЕФРОТИЧЕСКОГО СИНДРОМАУДЕТЕЙ: МУЛЬТИМАРКЕРНАЯ ПАРАЛЛЕЛЬНАЯ КАСКАДНАЯ СИСТЕМА
Цель исследования: изучить возможность использования результатов разных диагностических тестов у детей с нефротическим синдромом (НС) в процессе наблюдения в качестве прогностических маркеров. Материалы и методы. Операц...
АВТОМАТИЗОВАНЕ ОЦІНЮВАННЯ ФІБРОЗУ КОРКОВОГО ІНТЕРСТИЦІЮ З ВИКОРИСТАННЯМ ЗАБАРВЛЕННЯ СІРІУСОМ ЧЕРВОНИМ - РУТИННИЙ МЕТОД ДОСЛІДЖЕННЯ НЕФРОБІОПТАТІВ?
Цель работы – определить повторяемость и воспроизводимость автоматизированного количественного оценивания фиброза коркового интерстиция на материале нефробиопсий при окрашивании сириусом красным в сравнении с традиционны...
System inflammation and protein energy wasting correction in patients with chronic kidney disease
Protein-energy wasting as a manifestation of nutritional disorders is one of the central complications in chronic kidney disease (CKD) and largely determines quality and life expectancy. Both decrease in nutritional stat...
Comparative characteristic of the effect of anti-inflammatory therapy on the blood and urine levels of growth factors in patients with rheumatoid arthritis
The purpose of our work was to compare the effect of aceclofenac and meloxicam on the level of transforming growth factor-β1 (TGFβ1) and vascular endothelial growth factor in the blood and urine in chronic kidney disease...