Antioxidant properties of polyphenolic rich HPLC standardized extract of Beta vulgaris L. roots
Journal Title: International Journal of Research and Development in Pharmacy & Life Sciences (IJRDPL) - Year 2017, Vol 6, Issue 3
Abstract
OBJECTIVES: The present study was planned to evaluate the antioxidant effects of standardized Beta vulgaris L. or beetroot. METHODS: The polyphenols present in lyophilized water extract of beetroot (BR) were quantified and standardized by HPLC. Deactivation of free radicals by BR was studied by in vitro biochemical assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide and superoxide radical scavenging activities. Nitrite content of BR was estimated. Moreover, inhibition of protein denaturation properties of BR was carried out. RESULTS : HPLC chromatogram revealed presence of nine bioactive polyphenols in BR. Further, strong dose-dependent antioxidant properties were noted. IC50 (Inhibitory Concentration) of BR for DPPH, hydrogen peroxide and superoxide radical were 104.44µg/ml, 4.06µg/ml and 71.43µg/ml respectively. BR also showed enriched in nitrite content 3.359 mM/mg and it inhibited protein denaturation (IC50 594µg/ml). CONCLUSION: Beetroot was found to possess strong antioxidant property that may be due to presence of polyphenols.
Authors and Affiliations
Rania Indu| Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India, Anjan Adhikari| Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India, Moumita Ray| Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India, Alok K. Hazra| Chemistry Division, RKMA Quality Testing Laboratory (AYUSH), Narendrapur, South 24 Parganas, Kolkata, India, Tapas K. Sur| Department of Pharmacology, Institute of Post Graduate Medical Education & Research, 244, A.J.C. Bose Road, Kolkata, India, Anup Kumar Das| Department of Pharmacology, R.G. Kar Medical College, Kshudiram Bose Sarani, Kolkata, India
Keywords
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- EP ID EP16015
- DOI http://dx.doi.org/10.21276/IJRDPL.2278-0238.2017.6(3).2619-2
- Views 313
- Downloads 22
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