Antioxidants and Antimicrobial- Activities of Methanol Leaf Extract of Senna occidentalis
Journal Title: Journal of Advances in Medical and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 2
Abstract
Background: Oxidative stress and infectious disease have always been considered as a leading cause of morbidity and mortality in humans. Free radicals damage other molecules by extracting electrons from them in order to attain stability. The side effects associated with commonly used conventional antioxidants drugs have necessitated the search for natural antioxidants from plants extracts. Objective: The antioxidants and antimicrobial activities of methanol leaf extract of S. occidentalis obtained from North Central, Nigeria were evaluated against some pathogenic microorganism. Methods: The antioxidants activities were conducted using DPPH radical scavenging assay. The antibacterial activities were screened against Escherichia coli, Klebsiella pneumoniae and Candida albicans, Staphylococcus aureus, Pseudimonas aeruginosa and Salmonella typhi using agar well diffusion method at various concentrations (80-360 mg/ml). The minimum inhibitory concentration (MIC) was determined using serial dilution method while minimum bacterialcidal concentration (MBC) by plating various dilution of extract. Results: The extract show significant antibacterial activities with MIC in the range of 160 mg/ml- 280 mg/ml and MBC in range of 160 mg/ml – 320 mg/ml. The most sensitive organisms were S. aureus and P. aeruginosa while K. pneumonia show some degree of resistances compared to other organisms tested. However, the organisms were not sensitive to the extract at the dose of 80 and 120 mg/ml. The extracts and the standard antioxidant (Vitamin E) promoted an inhibition of DPPH radical with IC50 value of the extract (263.53±3.24 mg/mL) significantly (p < 0.05) higher than 83.63±2.78 mg/mL obtained for standard drug. Conclusion: Methanol leaf extract of S. occidentalis contains some useful potential antimicrobial and antioxidant compounds that could serve as candidate for the development of drug for therapeutic purposes.
Authors and Affiliations
Ndarubu A. Tsado, Bashir Lawal, Gwadabe N. Kontagora, Balarabe M. Muhammad, Makama A. Yahaya, Jiya A. Gboke, Umar A. Muhammad, Muhammad K. Hassan
Keywords
Related Articles
- EP ID EP328657
- DOI 10.9734/JAMPS/2016/25655
- Views 62
- Downloads 0
Effect of Various Preservatives on Pectin Base of Ofloxacin Vaginal GEL
The effect of various preservatives such as Methyl paraben, Propyl paraben, benzoic acid and Methyl paraben, Propyl paraben (1:10) on a pectin – base ofloxacin vaginal gel was tested. Five batches of pectin-based ofloxac...
Molecular Insights into the Role of Inflammation and Oxidative Stress in Epilepsy
Epilepsy is a chronic neurological disorder manifested as unpredictable, unprovoked recurrent seizures that affect a variety of mental and physical functions. Despite the use of current anti-epileptic drugs (AEDs) about...
Clinical Use and Complications of Peripheral Venous Cannulae among Adult Patients in a Nigerian Tertiary Hospital, Benin City: A Pilot Study
Background: Peripheral venous catheterization is a common medical procedure among hospitalized patients. Its use is fraught with potential complications including thrombophlebitis and blood stream infections. This necess...
Contextual Analysis and Perceptions of Health Workers on Migration: Perspective from Kenyatta National Hospital Staff, Nairobi Kenya
Aims: Migration of health workers constitutes a significant barrier to expanding health interventions. In Kenya, internal migration of workers, from rural to urban areas, is just as serious as international migration. He...
Oxytocin Hormone Administration Attenuates Cardiac Oxidative Injury and Inflammation in Hypercholesterolemic Rats
Aims: Oxytocin (OT) hormone has been recently recognized as a cardiovascular hormone with diverse regulatory roles. The present study examined the potential protective effect of exogenous OT administration on hypercholes...