ANTIOXIDATIVE AND ANTIBACTERIAL POTENTIALS OF FUNGAL ENDOPHYTES FROM JUSTICIA WYNAADENSIS HEYNE: AN ETHNOMEDICINAL RAIN FOREST SPECIES OF WESTERN GHATS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 6
Abstract
Objective: Endophytes, living within the medicinal plants, are recognized as an alternative source of bioactive components useful for human health. This study aims to isolate and identify the fungal endophytes from the ethnomedicinal rain forest plant Justicia wynaadensis and evaluation of their antioxidant and antimicrobial potentials. Methods: Endophytic fungi were isolated and identified by sequencing the internal transcribed spacer. The secondary metabolites were extracted with ethyl acetate and evaluated for the total phenolic, flavonoid, antioxidant, and antibacterial potentials. The antioxidative capacities were determined using different experimental models, viz., radical scavenging capacity, reducing power, and inhibition of lipid peroxidation. The antibacterial potential of extracts was determined through disc diffusion method and by evaluating minimum inhibitory concentration through microplate technique. Results: A total of nine fungal endophytes belonging to six genera were identified. The total phenolic content of the extracts ranged from 4.8±0.08 to 20.74±0.96 mg Gallic acid equivalent/g dry extract. Flavonoid was present in eight extracts in the range of 2.1±0.08 to 8.75±0.6 mg catechin equivalent/g dry extract. Fusarium incarnatum was found to have potentially high antioxidant capacity as well as broad spectrum antibacterial activity against Gram-positive and Gram-negative bacteria. Conclusion: This study reported various endophytic fungi from the plant J. wynaadensis. This is the first attempt to explore the treasure of endophytes and their bioactive potentiality from this plant. Hence, our findings encourage the exploration of these fungi and exploit them in search of potential drug discovery.
Authors and Affiliations
Madhuchhanda Das, Harischandra Sripathy Prakash, Monnanda Somaiah Nalini
Keywords
Related Articles
- EP ID EP605334
- DOI 10.22159/ajpcr.2017.v10i6.18106
- Views 128
- Downloads 0
PRELIMINARY PHYTOCHEMICAL SCREENING AND GAS CHROMATOGRAPHY AND MASS SPECTROMETRY ANALYSIS OF CHLOROFORM EXTRACT OF GARCINIA CAMBOGIA FRUITS
Objective: Garcinia cambogia fruit commonly known as Malabar tamarind, belongs to the family Clusiaceae, is said to show varied medicinal effects including antiobesity, antiviral, antimicrobial, anti-inflammatory, and an...
FORMULATION AND EVALUATION OF IN-SITU GEL CONTAINING CIPROFLOXACIN HYDROCHLORIDE IN THE TREATMENT OF PERIODONTITIS.
Objective: The present study describes the use of in-situ gel in periodontal drug delivery systems which contains gellan gum (0.4–0.6% w/v), pluronic F127 (14, 15 and 16% w/v), and drug Ciprofloxacin HCl (0.1% w/v). Nu...
ACACIA SENEGAL BARK EXTRACT IN REGULATION OF HYPOTHYROIDISM: AN EXPERIMENTAL APPROACH
Objective: The objective of the present study was to evaluate the thyroid hormone stimulating efficacy of Acacia senegal (Gum Arabic) bark extract in 6-n-propyl-2-thiouracil (PTU)-induced hypothyroid albino rats.Methods:...
P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS
Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.Methods: After informed consent, 96 Sudanese pregnant wome...
MOLECULAR EVALUATION OF ANTISTAPHYLOCOCCAL SECONDARY METABOLITES OF BACTERIAL ISOLATES FROM HOSPITAL WASTEWATER ENVIRONMENT
Objective: Antibiotics revolutionized medicine in the 20th century, and have together with vaccination led to the near eradication of diseases such as tuberculosis in the developed world. Their effectiveness and easy acc...