Antioxidative and neuroprotective activities of peanut sprout extracts against oxidative stress in SK-N-SH cells
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 1
Abstract
Objective: To evaluate the protective effect of peanut sprout extract (PSE) against paraquat (PQ) induced SK-N-SH cells. Methods: Three groups of cells were used in the experiment, together with a fourth, control group. One group was treated with PQ, the second group was treated with PSE, and the third group was pre-treated with PSE. The control group was untreated. Cell viability and toxicity were detected by MTT assay, cellular reactive oxygen species (ROS) was detected by Muse Cell Analyzer, quantitative RT-PCR was applied to investigate the expression of SIRT1 and a-synuclein genes, and Ab42 was detected by western blot. Results: The 50% effective concentration of PQ was 0.75 mmol/L. PSE had no significant cytotoxicity at a concentration of 1.5 mg/mL. In the group of cells pre-treated with PSE, cell death was significantly inhibited. In the PQ treated group, PQ was increased in the intracellular ROS in the cells. Intracellular ROS was significantly decreased in the cells treated with PSE and also those pre-treated with PSE. PSE significantly downregulated the expression of SIRT1 and a-syn genes, and it was found that PQ significantly increased b-amyloid 42 levels whereas this action was inhibited by PSE. Conclusions: PSE has neuroprotective activities against oxidative stress in SK-N-SH cells induced by PQ, suggesting that PSE is a highly promising agent in the prevention of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. 1. Introduction Neurodegeneration is the progressive loss of structure or function of neurons, including the death of neurons. Many neurodegenerative diseases including Alzheimer's disease (AD) and Parkinson's disease (PD) occur as a result of neurodegenerative processes [1]. AD has been defined by the presence of extracellular amyloid-b (Ab) containing plaques and cytoplasmic neurofibrillary tangles (NFT) consisting of abnormal microtubule associated protein tau. These proteinaceous aggregates are accompanied by synapse loss and neuronal cell death, which are thought to subserve the clinical syndrome of progressivecognitive impairment in AD [2,3]. PD is associated with progressive loss of dopaminergic neurons in the substantia nigra, as well as with more widespread neuronal changes that cause complex and variable motor and non-motor symptoms [4]. It is found that a-synuclein is a major component of Lewy bodies and Lewy neurites, the pathological hallmarks of PD, indicating its role in PD pathogenesis [5]. Environmental factors are important contributory factors in neurodegenerative disease [6]. Paraquat (PQ) (1,1-dimethyl-4,40-bipyridinium dichloride) is a widely used herbicide. It has been suggested that PQ might be an environmental factor contributing to neurodegenerative disorder *Corresponding author: Damratsamon Surangkul, Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand. Tel: +66 84 6881204 Fax: +66 55 964770 E-mail: damratsamon@gmail.com Foundation Project: Supported by National Research Council of Thailand (Grant No. R2558B114). Peer review under responsibility of Hainan Medical University. The journal implements double-blind peer review practiced by specially invited international editorial board members. HOSTED BY Contents lists available at ScienceDirect Asian Pacific Journal of Tropical Biomedicine journal homepage: www.elsevier.com/locate/apjtb 64 Asian Pac J Trop Biomed 2017; 7(1): 64–69 2221-1691/
Authors and Affiliations
Pranee Lertkaeo, Apinun Limmongkon, Metawee Srikummool, Tantip Boonsong, Wisa Supanpaiboon, Damratsamon Surangkul
Keywords
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- DOI 10.1016/j.apjtb.2016.11.007
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