Antiplasmodial activity of silver nanoparticles: A novel green synthesis approach
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2018, Vol 8, Issue 5
Abstract
Objective: To synthesize silver nanoparticles using silver nitrate by a green technique which involves different compositions of aqueous leaf extracts of Azadirachta indica (neem) and Ocimum sanctum (tulsi). Methods: Their shape and size were determined using transmission electron microscopy and UV-visible spectroscopy. Their antiplasmodial activity was studied using the malarial parasite strain (Plasmodium falciparum, 3D7). The parasite strain (3D7) was collected and revived in vitro using Trager and Jensen method in RPMI 1640 medium for 7-8 cycles. Half maximal effective concentration values were calculated by nonlinear regression analysis. Results: Transmission electron microscopy results confirmed the formation of silver nanoparticles with size ranging from 4.74-39.32 nm and their size differs by varying the concentrations from 20% to 100% of neem extract in neem and tulsi extracts. It was observed that samples B and C showed half maximum effective concentration of about 0.3 毺M. Conclusions: It can be easily established that the aqueous leaf extracts of neem and tulsi in combination can be a good source for synthesis of silver nanoparticles with small size possessing appreciable antiplasmodial activity.
Authors and Affiliations
Kumar Sanjay
Keywords
Related Articles
- EP ID EP369567
- DOI 10.4103/2221-1691.233008
- Views 52
- Downloads 0
Antioxidant activity and total phenolic and flavonoid content of the extract and chemical composition of the essential oil of Eremostachys laciniata collected from Zagros
Objective: To examine the chemical component of the essential oil of Eremostachys laciniata and evaluate antioxidant activity of the extract. Methods: The hydrodistillated essential oil was analyzed by gas chromatography...
Antidiabetic and hypolipidemic activities of Kigelia pinnata flowers extract in streptozotocin induced diabetic rats
Objective To evaluate antidiabetic and hypolipidemic activities of Kigelia pinnata methanolic flowers extract in streptozotocin (STZ) induced diabetic wistar rat. Methods Rats were made diabetic by a single dose of ST...
First survey of hard ticks (Acari: Ixodidae) on cattle, sheep and goats in Boeen Zahra and Takistan counties, Iran
Objective To carry out the distribution survey of hard ticks of livestock in Boeen Zahra and Takistan counties of Qazvin province from April 2010 to September 2010. Methods Nearly about 2 638 sheep, 461 goats and 318...
Chemical profile and antimicrobial activity of Secondatia floribunda A. DC (Apocynaceae)
Objective: To establish the chemical profile, and to evaluate the antibacterial and modulatory activity of the ethanolic extracts of the stalk's inner bark and heartwood of Secondatia floribunda. Methods: Quantification...
Protective effect of ashwagandha (Withania somnifera) against neurotoxicity induced by aluminum chloride in rats
Objective: To evaluate the neuroprotective effect of ashwagandha extract against aluminum chloride-induced neurotoxicity in rats. Methods: Rats were divided into control, aluminumintoxicated rats treated daily with alumi...