Antiradical Activity, Total Phenolic and Flavonoid Content of Extracts from the Stem Bark of Pterocarpus erinaceus
Journal Title: Scholars Academic Journal of Biosciences - Year 2016, Vol 4, Issue 6
Abstract
Abstract:In this study, radical scavenging activity, total phenolic and flavonoid contents of hexane, ethyl acetate, n-butanol and methanol extracts of the stem bark of Pterocarpus erinaceus were investigated. Antioxidant activity was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-benzothiazoline-6-sulfonic acid) (ABTS) assay. Total phenolic and flavonoid contents were evaluated with Folin-Ciocalteu and aluminium chloride colorimetric method respectively. Methanol extract showed the best antioxidant activity with the highest total flavonoid content (396.61±3.48 mgE Cat/1g DE) followed by n-butanol extract (263.63±5.44 mgECat/1g DE) and ethyl acetate extract (224.71±7.32 mgECat/1g DE). However, the n-butanol extract showed a highest phenolic content (490.03±4.02 mgEAA/1g DE) than the methanol extract (450.50±1.52 mgEAA/1g DE). These findings showed increase in antioxidant activity directly proportional to increase in extracting solvent’s polarity and flavonoid content. Keywords:Pterocarpus erinaceus, total phenolics, total flavonoid, radical scavenging, antioxidant activity
Authors and Affiliations
Paul Toukam Djouonzo, Christelle Flora Ladoh- Yemeda, Alembert Tiabou Tchinda, Joseph Tanyi Mbafor
Keywords
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