Antitrypanosomal Effects of Brassica Oleracea (Cabbage) Fruits and Leaves
Journal Title: Journal of Veterinary Advances - Year 2012, Vol 2, Issue 7
Abstract
In our continuation of search for antitrypanosomal agents from medicinal plants and other sources, Brassica oleracea (cabbage) fruits and leaves were screened for their antitrypanosomal and cytotoxicity effects. Methanolic plant extracts (MPES) of B. Oleracea fruits and leaves at different concentrations (250-1000 µg/ml) were tested against Trypanosoma evansi on Vero cell line grown in Dulbecco’s Modified Eagle Medium (DMEM) with foetal calf serum (FCS) (20-40%) at appropriate conditions. In vivo infectivity test of incubated MPES of B. Oleracea fruits, leaves and medium with trypanosomes were done in mice. In vitro cytotoxicity of the test extracts at concentrations (1.56-100 µg/ml) were performed on Vero cells but without FCS. Both MPES of B. oleracea fruits and leaves demonstrated trypanocidal activity, which ranged from immobilization, reduction and to the killing of trypanosomes. At 500 µg/ml of MPES of B. oleracea fruits and leaves with trypanosomes undergoing incubation, there were marked reductions of trypanosomes (19.33±0.33) (15.33±0.33) in the corresponding ELISA plate wells. But at 1000 µg/ml of MPE of B. oleracea fruits, there was no complete killing of the trypanosomes (5.33±0.33) as to that of MPE of leaves where trypanosomes could not be detected at 7 h of incubation, which was statistical comparable to the standard drug, diminazine aceturate at concentration of 50 µg/ml at 4 h of incubation. Trypanosomes counts decreased in concentration and time–dependent manner with significant difference (P<0.05). In in vivo infectivity test, group of mice inoculated with contents of ELISA plate wells with apparently killed trypanosomes survived for more than 30 days. While, the other group of mice inoculated with contents of ELISA plate wells with reduced trypanosomes in the incubated medium died of parasitaemia. MPES of B. oleracea fruits, leaves and diminazine aceturate (Berenil) were cytotoxic to Vero cells at all concentrations except at 1.56, 1.56 and 6.25-1.56 µg/ml, respectively.
Authors and Affiliations
P. Shaba , N. N. Pandey , O. P. Sharma , J. R. Rao , R. K. Singh
Keywords
Related Articles
- EP ID EP136021
- DOI -
- Views 110
- Downloads 0
Effect of Selenium on the Susceptibility of Vaccinated Cockerels against Infectious Bursal Disease
Infectious bursal disease (IBD) is rated as one of the most prevalent disease of commercial poultry in Nigeria. Outbreaks of IBD in spite of vaccination are a common occurrence in poultry production in Nigeria. An experi...
Prevalence of Mycobactrium Bovis Infection in Cattle Based on Intra-Dermal Caudal Fold Tuberculin Test in Adamawa State, Nigeria
A cross sectional survey was carried out using convenient sampling technique to sample 79 herds comprising of 2, 810 cattle in 5 Local Government Areas (L.G.A) of Adamawa State in the North East of Nigeria. This was base...
Antibiotic use, Abuse and their Public Health Implication: The Contributory role of management Flaws in the Poultry Industry in two AgroEcological Zones in Ghana
Fifty randomly selected registered commercial poultry farms in two agro-eccological zones in Ghana were investigated for contributory factors for antibiotic use and abuse and the public health implications thereof by mea...
Isolation and Molecular Characterization of Foot and Mouth Disease SAT2 Virus during Outbreak 2012 in Egypt
During April and May 2012, six outbreaks of FMD type SAT 2 were reported in Egyptian governorates: EL- Gharbiya (Kafr Qeretna, Al – Mahala Alkobra, Ebshaway Almalak, Qutoor), local cattle, buffaloes and dairy farms in Ka...
Identification of Toxoplasma gondii Excretory-Secretory Antigens from Propagation in Mice as Toxoplasmosis Vaccine Candidate
The aim of this research is to get some ESAs of T. gondii tachyzoite from propagation in mice and ESAs having the character of immunogenic as candidate of toxoplasmosis vaccine. The long-range target of this research is...