ANTITUMOUR ACTIVITY OF MESO-ZEAXANTHINE AND ITS MECHANISM OF ACTION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 5
Abstract
Objective: To evaluate the antitumour activity of meso-zeaxanthin and enumerate the mechanism of action.Methods: In vitro cytotoxicity was determined using transformed cells such as Dalton’s lymphoma ascites tumour cells, Ehrlich ascites tumour cells, L929 cells as well as using normal cells. Tumour reduction was determined by ascites tumour formation and solid tumour reduction. Mechanism of tumour reduction was determined by inhibition of apoptosis as seen by morphology, DNA ladder fraction and induction of P53 and caspase gene and inhibition of BCl2 gene.Results: Carotenoid meso-zeaxanthin was found to be toxic to Dalton’s Lymphoma ascites cells, Ehrlich ascites tumour cells and L929 cells (IC50, 46, 51 and 37 μg/ml respectively) and was not cytotoxic to normal cells. Meso-zeaxanthin increased the lifespan of animals bearing Ehrlich ascites tumour (69.9%) and decreased the solid tumour induced by Dalton’s Lymphoma ascites tumour. Meso-zeaxanthin was found to induce apoptosis to DLA cells as seen from DNA Lymphoma ascites tumour. Meso-zeaxanthin was found to induce apoptosis to DLA cells as seen from DNA fragmentation, and DNA laddering and up regulation of P [53] and caspase 3 and 9 down regulation of BCl2 gene expression.Conclusion: Meso-zeaxanthin which is non-toxic was found to reduce animal tumours.Keywords: Meso-zeaxanthin, Antitumour activity, Apoptosis, P[53] gene-BCl2geneÂ
Authors and Affiliations
Firdous A. P. , Binitha P. P, Girija Kuttan, Ramadasan Kuttan
Keywords
Related Articles
- EP ID EP577611
- DOI -
- Views 69
- Downloads 0
Perceptions of patients’ care givers regarding clinical pharmacists and their practice in a developing countryPERCEPTIONS OF PATIENTS’ CARE GIVERS REGARDING CLINICAL PHARMACISTS AND THEIR PRACTICE IN A DEVELOPING COUNTRY
Objective: The aim of the study was to report the perception of the care givers of the patients who have come across clinical pharmacists in their treatment course in a developing country like Pakistan.Methods: A cross s...
EFFECT OF ETHANOL-WATER COMPOSITION ON CLINDAMYCIN HYDROCHLORIDE PSEUDOPOLYMORPHISM
Objective: Formation of clindamycin hydrochloride (clindamycin HCl) in monohydrate-ethanolate from the recrystallization process with ethanol–water (5:2) has been reported a long time ago. However, the effect of ethano...
IMPACT OF tAI IN TRANSLATIONAL DYNAMICS OF HOMO SAPIENS GENES IN ESCHERICHIA COLI GENOME
Objective: Translation of mRNA to protein is a central biological process, and its regulation is vital for cell growth, development and differentiation. It is known that the tRNA molecules play a role of nearly 40% in th...
IN VITRO EVALUATION OF ANTIOXIDANT CAPACITY OF ALGERIAN ORIGANUM PLANT BY SPECTROPHOTOMETRICAL AND ELECTROCHEMICAL ASSAYS
Objective: The aim of the present study was to evaluate the antioxidant capacity and total phenolic of ethanolic extracts of two plants; Origanummajoranaand Origanumvulgare.Methods: Total phenolic was estimated by the Fo...
ANTIFUNGAL ACTIVITY OF CHLOROFORM AND ACETONE EXTRACTS OF SOLANUM DOLICHOSEPALUM AGAINST FUSARIUM OXYSPORUM
Objective: Evaluate the effectiveness of acetone (A) and chloroform (C) extracts of the plant Solanum dolichosepalum to control two isolated strains (S1 and S2), each from different specimen passiflora of the fungus Fusa...