Antiviral Activity Of Adamantane-Pyrazole Derivatives Against Foot And Mouth Disease Virus Infection In Vivo And In Vitro With Molecular Docking Study
Journal Title: Journal of Applied Veterinary Sciences - Year 2020, Vol 5, Issue 4
Abstract
Foot-and-mouth disease (FMD) vaccine does not afford early effective protection until adaptive immune protection caused by the vaccination occurs. Therefore, an alternative prophylactic application of antiviral agents for inhibition of the FMD virus is needed, and this is the scope of this study. In this study, we tested nine adamantane-pyrazole derivatives that could exhibit antiviral activity against FMDV infection either in vitro through baby hamster kidney cells (BHK-21 cells) infected with FMD virus serotypes O pan Asia. Cytotoxicity Concentration 50 (CC50) activity of pyrazole derivatives (1, 2, 3, 4, 5a,b, 6a-c) were detected on BHK-21 cells and ranged between 500 to 3000µg/ml. Inhibitory Concentration 50 (IC50) on BHK-21 was achieved only for the most promising three derivatives 6a-c and exhibited an antiviral activity with a therapeutic index of 30, and that was reflected on the antiviral activity response in baby mice with different concentrations where a concentration of 50 µg/ml for pyrazole derivatives 6a and 6c compounds and 40 µg/ml for bis-tolyl pyrazole 6b that achieve 100% protection and this results was as effective as 50 µg/ml of amantadine. Specifically, diaryl pyrazole derivatives 6a-c that protected for six days following FMDV challenge. These results suggested that pyrazole derivatives 6a-c could be used as an effective antiviral agent against FMD virus infection. Molecular docking simulation of the target compounds 6a-c had good binding energy and the tested compounds recommended being an excellent 3C protease inhibitor compared to Amantadine and Ribavirin. These findings may explain the antiviral activity of the target compounds.
Authors and Affiliations
Mohammed M. S. Wassel; Wael M. Gamal El-Din ; Ahmed Ragab; Gameel A. M. Elhag Ali and Yousry A. Ammar
Keywords
Related Articles
- EP ID EP689174
- DOI 10.21608/JAVS.2020.118001
- Views 176
- Downloads 0
Identification of Histopathological Changes Induced by Amitraz in Rats
Amitraz is used worldwide as a pesticide, it produces toxic effects when misused. The peresent study examined the histopathological changes of amitraz in the hepatocyte and kidney tissues of the laboratory albino rats. T...
Effects of Melatonin on Behavioural Activities in Acetaminophen-Induced Autism in Rat
The present study aims to investigate the effect of acetaminophen in male rats after early postnatal exposure to acetaminophen on several behavioural alterations and its role to precipitate autistic-like behaviour with i...
Histopathological and the Dose Depended Effects of Aspartame toxicity on Liver and Kidney of Rats
Aspartame is a commonly employed artificial sweetener used by hyperglycemic patients and in food management. Its danger depends on the outcome of previous studies that revealed controversy. The purpose of this study was...
Evaluation of Antibiotic Resistance Pattern of Escherichia Coli and Salmonella Species Isolated from Cloaca of Indigenous Chickens in Live-bird Markets in Marodi Jeh Region, Somalia
Poultry is an important source of protein globally, today, but Escherichia coli (E. coli)and Salmonella speciescontinue to be food-borne pathogens and contribute to the growing resistance to the antimicrobial agents. The...
Polycyclic Aromatic Hydrocarbons in Grilled Meats from Restaurants
Polycyclic aromatic hydrocarbons are a group of lipophilic compounds that can be generated during the preparation of food items at elevated temperatures. They are regarded as potentially genotoxic and carcinogenic to hum...