Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus
Journal Title: Asian Pacific Journal of Tropical Biomedicine - Year 2017, Vol 7, Issue 7
Abstract
Objective: To determine anti-viral activities of three Artocarpus species: Artocarpus altilis, Artocarpus camansi, and Artocarpus heterophyllus (A. heterophyllus) against Hepatitis C Virus (HCV). Methods: Antiviral activities of the crude extracts were examined by cell culture method using Huh7it-1 cells and HCV genotype 2a strain JFH1. The mode of action for anti-HCV activities was determined by time-of-addition experiments. The effect on HCV RNA replication and HCV accumulation in cells were analyzed by quantitative reverse transcription-PCR and western blotting, respectively. Results: The dichloromethane (DCM) extract of A. heterophyllus exhibited strong anti- HCV activity with an inhibitory concentration (IC50) value of (1.5 ± 0.6) mg/mL without obvious toxicity. The DCM extracts from Artocarpus altilis and Artocarpus camansi showed moderate anti-HCV activities with IC50 values being (6.5 ± 0.3) mg/mL and (9.7 ± 1.1) mg/mL, respectively. A time-of-addition studies showed that DCM extract from A. heterophyllus inhibited viral entry process though a direct virucidal activity and targeting host cells. HCV RNA replication and HCV protein expression were slightly reduced by the DCM treatment at high concentration. Conclusions: The DCM extract from A. heterophyllus is a good candidate to develop an antiviral agent to prevent HCV grant reinfection following liver transplantation.
Authors and Affiliations
Achmad Fuad Hafid, Chie Aoki-Utsubo, Adita Ayu Permanasari, Myrna Adianti, Lydia Tumewu, Aty Widyawaruyanti, Sri Puji Astuti Wahyuningsih, Tutik Sri Wahyuni, Maria Inge Lusida, Soetjipto Soetjipto, Hak Hotta
Keywords
Related Articles
- EP ID EP269196
- DOI 10.1016/j.apjtb.2017.06.003
- Views 38
- Downloads 0
Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG
Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis (M. tuberculosis) of a proteoliposome (PL) from Mycobacterium bovis Bacillus Calmette–Guerin...
Conocarpus erectus L., a plant with a high content of structural sugars, ions and phenolic compounds, shows antioxidant and antimicrobial properties promoted by different organic fractions
Objective: To evaluate the structural and chemical composition of plant and the antioxidant and antimicrobial activities promoted by hexanic, ethanolic and ethyl acetate fractions obtained from leaves of Conocarpus erect...
Sequence analysis of Turkish field strains of bovine torovirus shows unique amino acid changes in the partial M gene
Objective: To investigate the presence, prevalence and phylogenetic classification of bovine torovirus (BToV). Methods: Stool samples from 72 calves, which were negative for primary gastroenteritis agents, were examined...
Epidemiology, clinical features and transmission of re-emerging arboviral infection chikungunya
A number of re-emerging and emerging infectious diseases including chikungunya, West Nile, yellow fever, Zika, dengue, Japanese encephalitis, and others have increased in recent years, which threaten the public health ac...
In vitro assessment of the synergism between extracts of Cocos nucifera husk and some standard antibiotics
Objective: To evaluate the interactions between the crude extracts of Cocos nucifera (C. nucifera) and six front line antibiotics (ampicillin sodium salt, penicillin G sodium, amoxicillin, chloramphenicol, ciprofloxacin...