APOPTOSIS-INDUCING POTENTIAL OF LAWSONIA ALBA LAM. LEAVES ON HEPATOCELLULAR CARCINOMA (HEP-G2) CELLS ALONG WITH ITS ANTI-OXIDANT PROPERTY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 9
Abstract
Objective: The current study investigated the anti-cancer potential of methanolic and ethyl acetate fraction of Lawsonia alba L. (Lythraceae) leaves extract on Hep-G2 and RAW 264.7 cells along with in vitro anti-oxidant property of the ethyl acetate fraction.Methods: The cytotoxic activity of methanolic extract and its fractions had been studied by MTT assay on Hep-G2 and RAW 264.7 cells. Morphological study of Hep-G2 cells was performed by light, fluorescence and confocal microscope. 1% agarose gel electrophoresis, detection of apoptosis and cell cycle arrest by flow cytometric analysis had been performed to determine the proportion and stages of cellular apoptosis of Hep-G2 cells. In vitro anti-oxidant study of various fractions of MLA were performed by DPPH and Hydroxyl radical scavenging assay.Results: Cytotoxicity study of MLA and ELA had been confirmed by MTT assay and the IC50 value were calculated to be 75.85μg/ml and 32.81μg/ml on Hep-G2 cell line respectively. Morphological study showed the arrays of nuclear changes including chromatin condensation and apoptotic body formation indicating that treatment with ELA, causes apoptotic changes in the hepatoma cells compared to the untreated control. Agarose gel electrophoresis study showed fragmented DNA in the form of a ladder. Flow cytometric analysis showed an appreciable number of cells in early apoptosis stage. The cells were arrested, mostly in G0/G1 phase of the cell cycle. Antioxidant property of ELA fraction was confirmed by free radical scavenging activity.Conclusion: Ethyl acetate fraction of Lawsonia alba L. leaves possess potent apoptotic activity against Hep-G2 cell line along with notable anti-oxidant activity.
Authors and Affiliations
Soumyasree Dutta, Nilanjana Deb, Ashok Kumar Pattnaik, Shila Elizabeth Besra
Keywords
Related Articles
- EP ID EP576027
- DOI 10.22159/ijpps.2016.v8i9.12815
- Views 49
- Downloads 0
QUALITY EVALUATION AND SURVEY OF THE ESSENTIAL NEED FOR DRUG INFORMATION CENTERS
Objective: The first objective of this work was to examine the services provided by six drug information centers (DICs) in Alexandria, Egypt. The second objective was to evaluate the quality of the replies to the drug in...
EFFECT OF BLACK CUMIN (NIGELLA SATIVA) POWDER ON SERUM LIPID PROFILE, MALONDIALDHYDES, NITRITES, sICAM-1 AND sVCAM-1 IN EXPERIMENTALLY INDUCED ATHEROSCLEROSIS
Objective: Black cumin (Nigella Sativa), have been used for nutritional and medicinal purposes in many countries. This work was done to study the effect of black cumin powder during and after induction of atherosclerosis...
VALIDATION FOR QUANTITATIVE OF METHADONE ENANTIOMERS AND ITS MAJOR METABOLITE USING VANCOMYCIN COLUMN COUPLED WITH MASS SPECTROMETRIC DETECTION AND ITS APPLICATION TO CLINICAL SAMPLES
Objective: To develop method to measure both methadone enantiomers and its major metabolite 2-ethylidene-1, 5-dimethyl-3, 3-diphenylpyrrolidine (EDDP) in clinical samplesMethods: Five hundredmicroliters plasma/serum was...
ANTIBACTERIAL ACTIVITIES OF SCYTONEMA HOFMAN EXTRACTS AGAINST HUMAN PATHOGENIC BACTERIA
Objective: The present study is focused, to evaluate the effectiveness of organic solvent or aqueous extracts of Scytonema hofman against some human bacterial pathogens.Methods: The aqueous and organic solvent extracts o...
NEUROPROTECTIVE EFFECT OF CITRULLUS LANATUS SEED EXTRACTS ON CEREBRAL ISCHEMIC REPERFUSION INJURY INDUCED COGNITIVE IMPAIRMENT AND OXIDATIVE STRESS
Objective: Oxidative stress appears to be an early event involved in the pathogenesis of Alzheimer's disease (AD). The present study was designed to investigate the neuroprotective effects of citrullus lanatus on bilater...