Apoptosis of primary osteoblasts induced by dexamethasone: cellular and molecular mechanisms
Journal Title: Eklem Hastaliklari ve Cerrahisi - Year 2005, Vol 16, Issue 1
Abstract
Objectives: We investigated the effect of glucocorticoids on cell viability of osteoblasts and explored the cytological and molecular mechanisms of osteoblast apoptosis induced by glucocorticoids. Materials and methods: Dexamethasone was used to induce apoptosis of primary cultured osteoblast-like cells from skulls of suckling mouse. Surviving rate of osteoblasts, apoptosis rate, activity of caspase-3, and DNA binding activity of nuclear factor-kappa B were assessed using MTT-dye reduction microassay, flow cytometry, colorimetric substrate assay and electrophoretic mobility shift assay. The surviving rates of osteoblasts and fibroblasts were also compared to assess the differences in dexamethasone-induced effects on cells. Results: Dexamethasone significantly decreased the survival rate of osteoblast-like cells through an apoptotic process, activated cellular caspase-3, and inhibited the activity of nuclear factor-kappa B, in a concentration- and time-dependent manner (p
Authors and Affiliations
Nirong Bao, Jianning Zhao, Yurong Wang, Sujia Wu, Xiaofeng Zeng, Junhua Wang
Keywords
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