Application and evaluation of human antibodies in ABO grouping
Journal Title: Chinese Journal of Blood Transfusion - Year 2023, Vol 36, Issue 10
Abstract
Objective To evaluate the application of monoclonal typing reagents and human anti-A/B antibodies for absorption-elution test in ABO grouping. Methods The specificity of monoclonal typing reagents and human anti-A/B antibodies with standard A, B, O and AB phenotypes at 4 ℃, room temperature, and 37 ℃ were compared. Affinity was evaluated by the titer, agglutination time and agglutination intensity of the reaction with A1/B cells. 29 samples with ABO discrepancy were tested to evaluate the ability of monoclonal typing reagents and human anti-A/B antibodies to detect weak antigens in absorption-elution test. Results The specificity and affinity of human anti-A/B antibodies are low, and monoclonal typing reagents have cross reactivity. Human anti-A/B antibodies can detect most weak antigens in absorption-elution test with no cross reactivity. Conclusion In ABO grouping, the human anti A/B antibody binding absorption-elution test can serve as a supplement method for identifying ABO weak antigens. Accurate results can be obtained with reasonable reagents and corresponding methodology in serological tests,thus ensuring the safety of blood transfusion.
Authors and Affiliations
Qinqin ZUO, Liangzi ZHANG, Dazhou WU, Yong ZHANG, Xiaoyue CHU, Weiwei ZHANG, Hong WANG, Juan MAO, Hua XU
Keywords
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- EP ID EP738667
- DOI 10.13303/j.cjbt.issn.1004-549x.2023.10.016
- Views 45
- Downloads 0
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