Application of high performance thin layer chromatography method for ophthalmological preparations containing anthocyanins fractions
Journal Title: Journal of Pre-Clinical and Clinical Research - Year 2007, Vol 1, Issue 1
Abstract
The aim of the investigation was to elaborate a fast, easy, and cheap method of analysis of ophthalmological preparations containing anthocyanins fractions. A high performance thin layer chromatography (HPTLC) was applied; the chromatographic conditions were determined experimentally. Eight preparations with extracts of [i]Vaccinium myrtillus L[/i]. and [i]Aronia melanocarpa L.[/i] as reference material were analyzed. In the optimal chromatographic system, with methyl tert-butyl ether as the modifier of mobile phase, the separation of anthocyanins fractions was very good. The mobile phase enables separation of natural anthocyanins fractions from synthetic pigments (e.g., methylene blue).
Authors and Affiliations
Agnieszka Skalska-Kamińska, Anna Matysik, Marek Gerkowicz, Grażyna Matysik, Piotr Niedziela
Keywords
Related Articles
- EP ID EP58564
- DOI -
- Views 115
- Downloads 0
Effect of N-(p-ethoxycarbonylphenylmethyl) -p-isopropoxyphenylsuccinimide on the anticonvulsant action of four classical antiepileptic drugs in the mouse maximal electroshock-induced seizure model
[b]Introduction and objective[/b]. The purpose of this study was to determine the effects of N-(p-ethoxycarbonylphenylmethyl)-p-isopropoxyphenylsuccinimide (ECPM-IPPS), a new succinimide derivative, on the protective act...
Patient care after heart and kidney transplantation – case report
A patient after vascularized organ transplantation requires especially attentive medical care because of the risk of rejection of the transplant, the necessity for monitoring immunosuppressive therapy, and increased susc...
Epidemiology of non-invasive Aspergillosis of the maxillary sinuses – Clinical data from the Maxillofacial Surgery Clinic of the Medical University in Lublin, Poland, 2005–2014
Fungi are organisms which occur in the human environment. One of the potential pathogenic fungi is Aspergillus which belongs to mould, and is an etiological factor of non-invasive fungal paranasal sinusitis. Objective....
A novel biocompatibility test for disperse materials
[b]Introduction[/b]. Development of novel biocompatibility tests represents an urgent problem. Use of chicken embryos minimizes the effect of exogenous factors on the experimental course (as the chicken embryo develops i...
Hobnail hemangioma of the skin in a juvenile patient
Hobnail hemangioma is a rare, benign, vascular lesion with peculiar but commonly misleading morphology, located mostly on the extremities or trunk, and affects young and middle-aged adults with a slightly male predominan...