Application of multi-shoots cultures in micropropagation of willow herb (Chamaenerion angustifolium (L.) Scop.)
Journal Title: Herba Polonica - Year 2016, Vol 62, Issue 3
Abstract
Introduction: Willow herb (Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L. from Onagraceae family is a valuable medicinal plant that has been used in the treatment of urogenital disorders including BPH (Benign Prostatic Hypertrophy). The raw material is a rich source of polyphenols as well as steroids, triterpenoids and fatty acids. Due to frequent interspecific hybridization, plants collected in wild display a very diverse and variable content of active compounds. This poses a challenge in obtaining high quality and homogenous raw material. Application of the in vitro cultures and micropropagation techniques may offer a solution for alternative methods of cultivation. Objective: This work presents preliminary results of the first implementation of in vitro cultures of willow herb to obtain material for medicinal purposes. Methods: Sterile seedlings were donors of explants, which were used for induction of multi-shoots culture according to a modified protocol described by Turker and co-workers. Statistical analysis was used for assessment of significance of differences among variables. Results: Six different genotypes (lines) originating from root explants were chosen for clonal propagation. Efficiency of the elaborated method was 16–20 shoots per explant. Finally, over 3000 acclimatized plants were obtained and used for field crops. Conclusions: The use of Ch. angustifolium in vitro cultures can contribute to the introduction of this valuable herb for field crops and increase the availability of the raw material for food and pharmaceutical industries.
Authors and Affiliations
Mariola Dreger, Jolanta Wegenke, Joanna Makowiecka, Tomasz Michalik, Karolina Wielgus
Keywords
Related Articles
- EP ID EP333403
- DOI 10.1515/hepo-2016-0015
- Views 74
- Downloads 0
Influence of storage and pre-sowing treatment of southern sweet-grass seeds on their germination and initial growth of seedlings
Introduction: Southern sweet-grass (Hierochloë australis /Schrad./ Roem. et Schult., Poaceae) is a perennial tuft-grass occurring in North-Eastern part of Europe. Its leaves are collected from wild growing plants as an a...
Chemical diversity within strawberry (Fragaria vesca L.) species
The chemical diversity within wild strawberry (Fragaria vesca L.) species was studied depending on its origin (natural vs cultivated). The herb was collected at the full flowering stage. Following items were determined...
The antimicrobial and antioxidant activities of extracts obtained from some moss species in Turkey
Introduction: Pharmaceutical industry is forced to develop new pharmacologically active molecules. Like other plants, mosses are considered to be potential source of new biologically active compounds. Objective: The pres...
The effect of colour grading of milk thistle (Silybum marianum (L.) Gaertn.) seeds on their quality for sowing
Introduction: Milk thistle (Silybum marianum (L.) Gaertn.) is a medicinal plant belonging to Asteraceae family. Extract from milk thistle achenes (termed in practice as seeds) contains sylimarin, which protects liver cel...
Cichorin A: a benzo-isochromene from Nypa fruticans endophytic fungus Pestalotiopsis sp.
Introduction: Similar bioactive metabolites are obtainable from host plants as well as the endophytic fungi residing in them. Objective: The aim of the study is to isolate the major compound(s) from the endophytic fungus...