APPLICATION OF PLANT ESSENTIAL OILS FOR IMPROVING AIR QUALITY IN THE LECTURE-HALLS OF UNIVERSITIES
Journal Title: Вісник Національного Авіаційного Університету - Year 2018, Vol 75, Issue 2
Abstract
Purpose: The general goal of the article is to analyze air contamination of lecturer-halls by microorganisms and to investigate antimicrobial activity of plant essential oils for improvem ent of ecological state of indoor microflora. Methods: For determination of the total number of microorganisms and assessment of air microflora contamination, Sedimentation method (Koch’s Pour Plate method) was used. The colonies of microorganisms were calculated accord ing to the V. Omelianskii rule. Sta tistical processing of data made on the Microsoft Office Exel (with adequacy 95%). Indi cators of standard deviations were calculated by conventional procedures. The morphology of microorgani sms was studied on microscope "Mikmed 2" (with zoom × 1500). The impact of plant essential oils on chosen strains of microorganisms (Staphylococcus aureus UCM B-904, Pseudomonas aeruginosa UCM В -907, Escherichia coli UCM B-906) was estimated with the help of disc-diffusion method. Results: The round, white, yellow with a matte or shiny surface and with a smooth edge of the colonies of microorganism s were identified during the investigation. By the morphological features, microorganisms were gram-ne gative bacilli or gram-positive cocci. It has been established that the efficiency of plant essential oils appears in small concentrations and during of its increasing it changes insignificantly. In th e research of antimicrobial activity of essential oils with the help of disco-diffusion method, it has been determined that the essential oils of lavender and eucalyptus have antimicrobial activity against to all the strains investigated. Conclusions: It has been investigated that investigated essential oils (lavender, eucalyptus, and juniper) inhibit the microorganisms to increase in the concentrations 5-15 mcl/m3 and can be used as the effective method of ai r treatment. Based on the research, the essential oils of lavender and eucalyptus can be reco mmended to use after cla sses in the concentrations of 5 mcl/m 3 .
Authors and Affiliations
Tetiana Bilyk, Nataliia Lukianenko, Karina Vityk, Olesia Havryliuk
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