APPLICATION OF UV SPECTROPHOTOMETERIN METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANIOUS ESTIMATION OF GABAPENTIN AND MECOBALAMINE IN PHARMACEUTICAL DOSAGE FORM
Journal Title: World Journal of Pharmaceutical Research - Year 2018, Vol 7, Issue 7
Abstract
Two precise, simple, accurate, reproducible, rapid and economical UV spectrophotometric methods have been developed for simultaneous estimation of gabapentine and mecobalamine in tablet dosage form by using 0.1N HCL as solvent. Method I is based on formation and solving of simultaneous equation method, known as vierodt’s method. Gabapentine and mecobalamine show absorbance maxima at 283 nm and 274 nm respectively, so absorbance was measured at the same wavelength for the estimation of gabapentine and mecobalamine in tablet combination. Method II is based on principle of Q-analysis, known as absorbance ratio method. Absorbance was measured at 285 nm (isobestic point) and 274 nm (λmax of Methylcobalamin.). gabapentine and mecobalamine obeys beer’s law in the concentration range of 5 to 30 μg/ml. Percentage estimation of gabapentine and mecobalamine in tablet dosage form by method I is 99 and 99.85 and by method II is 99 and 99.89 respectively with standard deviation ≤2. Methods are validated according to ICH guidelines and can be applied for routine analysis of drugs in tablet dosage form.
Authors and Affiliations
Sonali T. Gade
Keywords
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